Molecular, serological and in vitro culture-based characterization of Bourbon virus, a newly described human pathogen of the genus Thogotovirus

Details

• Alternative Title:
  J Clin Virol
• Personal Author:
  Lambert, Amy J. ; Velez, Jason O. ; Brault, Aaron C. ; Calvert, Amanda E. ; Bell-Sakyi, Lesley ; Bosco-Lauth, Angela M. ; Staples, J. Erin ; Kosoy, Olga I.
• Description:
  Background
  
  In June of 2014, a previously healthy man from Kansas with a recent history of tick exposure died from complications related to an illness marked by fever, thrombocytopenia and leukopenia. An isolate was derived from the blood of this patient during the course of diagnostic testing. This isolate was subsequently identified as a novel orthomyxovirus of the genus Thogotovirus by next generation sequencing and was named Bourbon virus after the patient’s county of residence.
  
  Objectives
  
  To support research and diagnostic aims, we provide a basic description of Bourbon virus at both the molecular and serological levels. Furthermore, to preliminarily identify potential host and vector range associations we have characterized the growth kinetics of Bourbon virus in a variety of vertebrate and invertebrate cell lines.
  
  Study design
  
  Bourbon virus was subjected to next generation-high throughput sequencing, phylogenetic, and basic structural protein analyses as well as 2-way plaque reduction neutralization assays. Also, we inoculated a variety of cell types with Bourbon virus and evaluated the growth kinetics by determining viral titers in the supernatants taken from infected cells over time.
  
  Results
  
  Bourbon virus possesses 24–82% identity at the amino acid sequence level and low serological cross-reactivity with other Thogotoviruses. In vitro growth kinetics reveal robust replication of Bourbon virus in mammalian and tick cells.
  
  Conclusions
  
  Molecular and serological characterizations identify Bourbon virus as a novel member of the genus sThogotovirus. Results from cell culture analyses suggest an association between Bourbon virus and mammalian and tick hosts.
  
  
• Subjects:
  Animals Article Bourbon Virus Cell Line Characterization Chlorocebus Aethiops Disease Models, Animal Genome, Viral HeLa Cells Humans Influenza, Human In Vitro Growth Male Mice Molecular Phylogeny Serological Thogotovirus Vero Cells Viral Load

  More +
• Source:
  J Clin Virol. 73:127-132
• Pubmed ID:
  26609638
• Pubmed Central ID:
  PMC5683172
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  73
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:5dcd06b59dfc236fae3d0a4b7e5d31f103464dc7335e1511d8ae192a109d3e04
• Download URL:
  https://stacks.cdc.gov/view/cdc/49798/cdc_49798_DS1.pdf
• File Type:
  [PDF - 853.92 KB ]