Details:

Alternative Title:
J Clin Virol
Publisher's site:
http://dx.doi.org/10.1016/j.jcv.2015.10.021
Personal Author:
Lambert, Amy J. ; Velez, Jason O. ; Brault, Aaron C. ; Calvert, Amanda E. ; Bell-Sakyi, Lesley ; ... More +
Lambert, Amy J. ; Velez, Jason O. ; Brault, Aaron C. ; Calvert, Amanda E. ; Bell-Sakyi, Lesley ; Bosco-Lauth, Angela M. ; Staples, J. Erin ; Kosoy, Olga I. Less -
Description:
Background

In June of 2014, a previously healthy man from Kansas with a recent history of tick exposure died from complications related to an illness marked by fever, thrombocytopenia and leukopenia. An isolate was derived from the blood of this patient during the course of diagnostic testing. This isolate was subsequently identified as a novel orthomyxovirus of the genus Thogotovirus by next generation sequencing and was named Bourbon virus after the patient’s county of residence.

Objectives

To support research and diagnostic aims, we provide a basic description of Bourbon virus at both the molecular and serological levels. Furthermore, to preliminarily identify potential host and vector range associations we have characterized the growth kinetics of Bourbon virus in a variety of vertebrate and invertebrate cell lines.

Study design

Bourbon virus was subjected to next generation-high throughput sequencing, phylogenetic, and basic structural protein analyses as well as 2-way plaque reduction neutralization assays. Also, we inoculated a variety of cell types with Bourbon virus and evaluated the growth kinetics by determining viral titers in the supernatants taken from infected cells over time.

Results

Bourbon virus possesses 24–82% identity at the amino acid sequence level and low serological cross-reactivity with other Thogotoviruses. In vitro growth kinetics reveal robust replication of Bourbon virus in mammalian and tick cells.

Conclusions

Molecular and serological characterizations identify Bourbon virus as a novel member of the genus sThogotovirus. Results from cell culture analyses suggest an association between Bourbon virus and mammalian and tick hosts.


Subject:
[+]
Animals
Article
Bourbon Virus
Cell Line
Cercopithecus Aethiops
Characterization
Disease Models, Animal
Genome, Viral
HeLa Cells
Humans
Influenza, Human
In Vitro Growth
Male
Mice
Molecular
Phylogeny
Serological
Thogotovirus
Vero Cells
Viral Load

Source:
J Clin Virol. 73:127-132.
Pubmed ID:
26609638
Pubmed Central ID:
PMC5683172
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:5dcd06b59dfc236fae3d0a4b7e5d31f103464dc7335e1511d8ae192a109d3e04
File Type:

Supporting Files

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