Use of a Scalable Replicon-Particle Vaccine to Protect Against Lethal Lassa Virus Infection in the Guinea Pig Model

Details

• Alternative Title:
  J Infect Dis
• Personal Author:
  Kainulainen, Markus H. ; Spengler, Jessica R. ; Welch, Stephen R. ; Coleman-McCray, JoAnn D. ; Harmon, Jessica R. ; Klena, John D. ; Nichol, Stuart T. ; Albariño, César G. ; Spiropoulou, Christina F.
• Description:
  Lassa fever is a viral zoonosis that can be transmitted from person to person, especially in the hospital setting. The disease is endemic to several countries in West Africa and can be a major contributor to morbidity and mortality in affected areas. There are no approved vaccines to prevent Lassa virus infection. In this work, we present a vaccine candidate that combines the scalability and efficacy benefits of a live vaccine with the safety benefits of single-cycle replication. The system consists of Lassa virus replicon particles devoid of the virus essential glycoprotein gene, and a cell line that expresses the glycoprotein products, enabling efficient vaccine propagation. Guinea pigs vaccinated with these particles showed no clinical reaction to the inoculum and were protected against fever, weight loss, and lethality after infection with Lassa virus.
• Subjects:
  • Africa, Western
  • Animals
  • Cell Line
  • Chlorocebus aethiops
  • Disease Models, Animal
  • Guinea Pigs
  • Lassa Fever
  • Lassa virus
  • Replicon
  • Vaccines, Attenuated
  More +
• Keywords:
  • Lassa
  • vaccine
  • virus replicon particle
  • VRP
  • single-cycle replication
• Source:
  J Infect Dis. 217(12):1957-1966
• Pubmed ID:
  29800368
• Pubmed Central ID:
  PMC6086598
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  217
• Issue:
  12
• Download URL:
  https://stacks.cdc.gov/view/cdc/58092/cdc_58092_DS1.pdf
• File Type:
  [PDF - 1.28 MB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:e37f2df834308643e5882b74bd4c3bab53a667e64c43e01c8139f02866a6d7ed