Microtubules regulate brush border formation

Details

• Alternative Title:
  J Cell Physiol
• Personal Author:
  Tonucci, Facundo M. ; Ferretti, Anabela ; Almada, Evangelina ; Cribb, Pamela ; Vena, Rodrigo ; Hidalgo, Florencia ; Favre, Cristián ; Tyska, Matt J. ; Kaverina, Irina ; Larocca, Maria C.
• Description:
  Most epithelial cells contain apical membrane structures associated to bundles of actin filaments, which constitute the brush border. Whereas microtubule participation in the maintenance of the brush border identity has been characterized, their contribution to de novo microvilli organization remained elusive. Hereby, using a cell model of individual enterocyte polarization, we found that nocodazole induced microtubule depolymerization prevented the de novo brush border formation. Microtubule participation in brush border actin organization was confirmed in polarized kidney tubule MDCK cells. We also found that centrosome, but not Golgi derived microtubules, were essential for the initial stages of brush border development. During this process, microtubule plus ends acquired an early asymmetric orientation toward the apical membrane, which clearly differs from their predominant basal orientation in mature epithelia. In addition, overexpression of the microtubule plus ends associated protein CLIP170, which regulate actin nucleation in different cell contexts, facilitated brush border formation. In combination, the present results support the participation of centrosomal microtubule plus ends in the activation of the polarized actin organization associated to brush border formation, unveiling a novel mechanism of microtubule regulation of epithelial polarity.
• Subjects:
  Actin Cytoskeleton Animals Cell Polarity Centromere Colon Dogs Enterocytes Epithelial Cells Humans Kidney Madin Darby Canine Kidney Cells Microtubule-Associated Proteins Microtubules Microvilli Nocodazole Time Factors Tubulin Modulators

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• Keywords:
  Actin Brush Border Epithelial Polarity Microtubules MTOC
• Source:
  J Cell Physiol. 233(2):1468-1480
• Pubmed ID:
  28548701
• Pubmed Central ID:
  PMC5673559
• Document Type:
  Journal Article
• Funding:
  R01 DK111949/DK/NIDDK NIH HHSUnited States/ ; R01 DK095811/DK/NIDDK NIH HHSUnited States/ ; H75 IP000287/IP/NCIRD CDC HHSUnited States/ ; R01 DK106228/DK/NIDDK NIH HHSUnited States/ ; R01 DK075555/DK/NIDDK NIH HHSUnited States/ ; P30 DK058404/DK/NIDDK NIH HHSUnited States/ ; R01 GM078373/GM/NIGMS NIH HHSUnited States/
• Volume:
  233
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:cc72a7bded6e19a1e7f4f61bb34fa46fb414c499030172544c36e30e0afcaae6
• Download URL:
  https://stacks.cdc.gov/view/cdc/49650/cdc_49650_DS1.pdf
• File Type:
  [PDF - 970.54 KB ]