Structural organization of the dynein-dynactin complex bound to microtubules

Details

• Alternative Title:
  Nat Struct Mol Biol
• Personal Author:
  Chowdhury, Saikat ; Ketcham, Stephanie A. ; Schroer, Trina A. ; Lander, Gabriel C.
• Description:
  Cytoplasmic dynein associates with dynactin to drive cargo movement on microtubules, but the structure of the dynein-dynactin complex is unknown. Using electron microscopy, we determined the organization of native bovine dynein, dynactin and the dynein-dynactin-microtubule quaternary complex. In the microtubule-bound complex, the dynein motor domains are positioned for processive unidirectional movement, and the cargo-binding domains of both dynein and dynactin are accessible.
• Keywords:
  Animals Cattle Cryoelectron Microscopy Dynactin Complex Dyneins Microtubule-Associated Proteins Microtubules Models, Molecular
• Source:
  Nat Struct Mol Biol. 22(4):345-347
• Pubmed ID:
  25751425
• Pubmed Central ID:
  PMC4385409
• Document Type:
  Journal Article
• Funding:
  R01 GM044589/GM/NIGMS NIH HHSUnited States/ ; DP2 EB020402-01/DP/NCCDPHP CDC HHSUnited States/ ; DP2 EB020402/EB/NIBIB NIH HHSUnited States/ ; GM44589/GM/NIGMS NIH HHSUnited States/ ; R56 GM044589/GM/NIGMS NIH HHSUnited States/
• Volume:
  22
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:4683edf6af9e82f3e87556b8c9b3cb2a69ae063f1f3ec05d479b797b6f1bdf98
• Download URL:
  https://stacks.cdc.gov/view/cdc/31308/cdc_31308_DS1.pdf
• File Type:
  [PDF - 946.72 KB ]