i
Development of an oral fluid immunoassay to assess past and recent hepatitis E virus (HEV) infection
-
9 2017
-
-
Source: J Immunol Methods. 448:1-8
[PDF-522.07 KB]
Details:
-
Alternative Title:J Immunol Methods
-
Personal Author:
-
Description:Background
Hepatitis E virus (HEV) infection causes significant morbidity and mortality worldwide, particularly among pregnant women. In clinical settings blood-based testing protocols are commonly used to diagnose HEV infection, but in community settings such invasive sampling can hinder study participation and limit discovery of the ecology and natural history of HEV infection. Oral fluid is a non-invasive biospecimen that can harbor pathogen-specific antibodies and has the potential to replace blood-based testing protocols.
Objectives
To develop an immunoassay to assess past and recent HEV infection that uses oral fluid instead of serum or plasma.
Methods
The assay was validated using paired oral fluid and serum samples collected from 141 patients who presented either with (n=76) or without (n=65) symptoms of acute viral hepatitis at a clinical diagnostics center in Dhaka, Bangladesh. The sensitivity and specificity of the oral fluid-based immunoassay for HEV IgG (past HEV infection) and HEV IgA (recent HEV infection) antibodies was calculated in reference to Wantai’s (Beijing Wantai) serum-based HEV enzyme-linked immunosorbent assay (ELISA) kits for IgG and IgM antibodies, respectively.
Results
The sensitivity and specificity of the oral fluid-based immunoassay for HEV-IgG antibodies were 98.7% and 98.4%, respectively. The sensitivity and specificity of the oral fluid-based immunoassay for HEV IgA were 89.5% and 98.3%, respectively.
Conclusions
The high concordance of our non-invasive oral fluid-based immunoassays (HEV IgG and HEV IgA) with commercial high-performance serum HEV ELISA kits (IgG and IgM) means that population-based surveillance of past and recent HEV infection could be expanded to improve our understanding of its ecology and natural history.
-
Subjects:
-
Source:
-
Pubmed ID:28478117
-
Pubmed Central ID:PMC5597243
-
Document Type:
-
Funding:
-
Place as Subject:
-
Volume:448
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
