Details:

Alternative Title:
J Clin Microbiol
Publisher's site:
http://dx.doi.org/10.1128/JCM.00630-17
Personal Author:
Kordalewska, Milena ; Zhao, Yanan ; Lockhart, Shawn R. ; Chowdhary, Anuradha ; Berrio, Indira ; ... More +
Kordalewska, Milena ; Zhao, Yanan ; Lockhart, Shawn R. ; Chowdhary, Anuradha ; Berrio, Indira ; Perlin, David S. ; Less -
Description:
Candida auris is an emerging multidrug-resistant fungal pathogen causing nosocomial and invasive infections associated with high mortality. C. auris is commonly misidentified as several different yeast species by commercially available phenotypic identification platforms. Thus, there is an urgent need for a reliable diagnostic method. In this paper, we present fast, robust, easy-to-perform and interpret PCR and real-time PCR assays to identify C. auris and related species: Candida duobushaemulonii, Candida haemulonii, and Candida lusitaniae Targeting rDNA region nucleotide sequences, primers specific for C. auris only or C. auris and related species were designed. A panel of 140 clinical fungal isolates was used in both PCR and real-time PCR assays followed by electrophoresis or melting temperature analysis, respectively. The identification results from the assays were 100% concordant with DNA sequencing results. These molecular assays overcome the deficiencies of existing phenotypic tests to identify C. auris and related species.
Subject:
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Candida Auris
Candida Duobushaemulonii
Candida Haemulonii
Candida Lusitaniae
Diagnostics
Identification
Mycology
PCR
Real-time PCR

Source:
J Clin Microbiol. 2017 ; 55(8):2445-2452.;
Pubmed ID:
28539346
Pubmed Central ID:
PMC5527423
Document Type:
Journal Article ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:ee7218d8066ed348cd5d36ea956e28cf7d095b6ed675b2ecec5ef8b19810578e
File Type:

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