Rapid and Accurate Molecular Identification of the Emerging Multidrug-Resistant Pathogen Candida auris

Kordalewska, Milena; Zhao, Yanan; Lockhart, Shawn R.; Chowdhary, Anuradha; Berrio, Indira; Perlin, David S.

doi:10.1128/JCM.00630-17

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Rapid and Accurate Molecular Identification of the Emerging Multidrug-Resistant Pathogen Candida auris

Published Date:

May 24 2017

Publisher's site:

http://dx.doi.org/10.1128/JCM.00630-17

Source:

J Clin Microbiol. 2017; 55(8):2445-2452.

[PDF-931.82 KB]

Details:

Alternative Title:

J Clin Microbiol

Personal Author:

Kordalewska, Milena ; Zhao, Yanan ; Lockhart, Shawn R. ; Chowdhary, Anuradha ; Berrio, Indira ; ... More ▼

Description:

Candida auris is an emerging multidrug-resistant fungal pathogen causing nosocomial and invasive infections associated with high mortality. C. auris is commonly misidentified as several different yeast species by commercially available phenotypic identification platforms. Thus, there is an urgent need for a reliable diagnostic method. In this paper, we present fast, robust, easy-to-perform and interpret PCR and real-time PCR assays to identify C. auris and related species: Candida duobushaemulonii, Candida haemulonii, and Candida lusitaniae Targeting rDNA region nucleotide sequences, primers specific for C. auris only or C. auris and related species were designed. A panel of 140 clinical fungal isolates was used in both PCR and real-time PCR assays followed by electrophoresis or melting temperature analysis, respectively. The identification results from the assays were 100% concordant with DNA sequencing results. These molecular assays overcome the deficiencies of existing phenotypic tests to identify C. auris and related species.

Subject:

[+]

Pubmed ID:

28539346

Pubmed Central ID:

PMC5527423

Document Type:

Journal Article

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

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