Details:

Alternative Title:
Diagn Microbiol Infect Dis
Publisher's site:
http://dx.doi.org/10.1016/j.diagmicrobio.2016.03.022
Personal Author:
Cross, Kristen E. ; Mercante, Jeffrey W. ; Benitez, Alvaro J. ; Brown, Ellen W. ; Diaz, Maureen H. ; ... More +
Cross, Kristen E. ; Mercante, Jeffrey W. ; Benitez, Alvaro J. ; Brown, Ellen W. ; Diaz, Maureen H. ; Winchell, Jonas M. Less -
Description:
Legionnaires' disease is a severe respiratory disease that is estimated to cause between 8,000 and 18,000 hospitalizations each year, though the exact burden is unknown due to under-utilization of diagnostic testing. Although Legionella pneumophila is the most common species detected in clinical cases (80-90%), other species have also been reported to cause disease. However, little is known about Legionnaires' disease caused by these non-pneumophila species. We designed a multiplex real-time PCR assay for detection of all Legionella spp. and simultaneous specific identification of four clinically-relevant Legionella species, L. anisa, L. bozemanii, L. longbeachae, and L. micdadei, using 5'-hydrolysis probe real-time PCR. The analytical sensitivity for detection of nucleic acid from each target species was ≤50fg per reaction. We demonstrated the utility of this assay in spiked human sputum specimens. This assay could serve as a tool for understanding the scope and impact of non-pneumophila Legionella species in human disease.
Subject:
[+]
Article
DNA Primers
Humans
Legionella
Legionella Anisa
Legionella Bozemanii
Legionella Longbeachae
Legionella Micdadei
Legionnaires' Disease
Multiplex Polymerase Chain Reaction
Multiplex Real-time PCR
Oligonucleotide Probes
Real-Time Polymerase Chain Reaction
Sensitivity And Specificity

Source:
Diagn Microbiol Infect Dis. 85(3):295-301.
Pubmed ID:
27107536
Pubmed Central ID:
PMC5505572
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:5bd1c9e73612c89bcba84cb2c97baa04adefdf98867793ddbe38b2e8c1aa246c
File Type:

Supporting Files

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