Simultaneous detection of Legionella species and L. anisa, L. bozemanii, L. longbeachae and L. micdadei using conserved primers and multiple probes in a multiplex real-time PCR assay☆

Cross, Kristen E.; Mercante, Jeffrey W.; Benitez, Alvaro J.; Brown, Ellen W.; Diaz, Maureen H.; Winchell, Jonas M.

doi:10.1016/j.diagmicrobio.2016.03.022

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Simultaneous detection of Legionella species and L. anisa, L. bozemanii, L. longbeachae and L. micdadei using conserved primers and multiple probes in a multiplex real-time PCR assay☆

Published Date:

Apr 02 2016

Publisher's site:

http://dx.doi.org/10.1016/j.diagmicrobio.2016.03.022

Source:

Diagn Microbiol Infect Dis. 85(3):295-301.

[PDF-797.97 KB]

Details:

Alternative Title:

Diagn Microbiol Infect Dis

Personal Author:

Cross, Kristen E. ; Mercante, Jeffrey W. ; Benitez, Alvaro J. ; Brown, Ellen W. ; Diaz, Maureen H. ; ... More ▼

Description:

Legionnaires' disease is a severe respiratory disease that is estimated to cause between 8,000 and 18,000 hospitalizations each year, though the exact burden is unknown due to under-utilization of diagnostic testing. Although Legionella pneumophila is the most common species detected in clinical cases (80-90%), other species have also been reported to cause disease. However, little is known about Legionnaires' disease caused by these non-pneumophila species. We designed a multiplex real-time PCR assay for detection of all Legionella spp. and simultaneous specific identification of four clinically-relevant Legionella species, L. anisa, L. bozemanii, L. longbeachae, and L. micdadei, using 5'-hydrolysis probe real-time PCR. The analytical sensitivity for detection of nucleic acid from each target species was ≤50fg per reaction. We demonstrated the utility of this assay in spiked human sputum specimens. This assay could serve as a tool for understanding the scope and impact of non-pneumophila Legionella species in human disease.

Subject:

[+]

Pubmed ID:

27107536

Pubmed Central ID:

PMC5505572

Document Type:

Journal Article

Funding:

CC999999/Intramural CDC HHS/United States

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

File Type:

Supporting Files:

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Simultaneous detection of Legionella species and L. anisa, L. bozemanii, L. longbeachae and L. micdadei using conserved primers and multiple probes in a multiplex real-time PCR assay☆

Simultaneous detection of Legionella species and L. anisa, L. bozemanii, L. longbeachae and L. micdadei using conserved primers and multiple probes in a multiplex real-time PCR assay☆

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