Details:

Alternative Title:
Anal Bioanal Chem
Personal Author:
Zhang, Li ; Xia, Yang ; Xia, Baoyun ; Nicodemus, Keegan J. ; McGuffey, James ; ... More +
Zhang, Li ; Xia, Yang ; Xia, Baoyun ; Nicodemus, Keegan J. ; McGuffey, James ; McGahee, Ernest ; Blount, Benjamin ; Wang, Lanqing Less -
Description:
Heterocyclic aromatic amines (HCAA) are listed by the US Food and Drug Administration (FDA) as harmful or potentially harmful constituents of tobacco smoke. However, quantifying HCAA exposure is challenging. In this study, we developed a sensitive, precise, and accurate isotope dilution, liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify urinary HCAAs in smokers and nonsmokers. The high-throughput robotic sample preparation system could handle a throughput of over 300 samples per day, while maintaining intra-day and inter-day imprecision and bias ≤10 %. The limits of detection of carcinogenic HCAAs ranged from 0.31 to 0.83 pg/mL. The validated method was applied to measure HCAAs in urine collected from smokers and non-smokers. This sensitive and efficient analytical method is ideal to support large-scale biomonitoring studies of HCAA exposure. Graphical Abstract LC/MS/MS and robotic sample preparation system for urinary HCAA analysis.
Subjects:
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Article Automation Heterocyclic Aromatic Amines High Throughput LC-MS/MS Urine
Source:
Anal Bioanal Chem. 408(28):8149-8161.
Pubmed ID:
27687186
Pubmed Central ID:
PMC5460753
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:cabaa8d47c5fe63c300e9f961fe6310b2f0e720c5ec7590f19fd1faaba543db2
Download URL:
https://stacks.cdc.gov/view/cdc/46076/cdc_46076_DS1.pdf
File Type:

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