Details:

Alternative Title:
Toxicon
Personal Author:
Coleman, Rebecca ; Lemire, Sharon W. ; Bragg, William ; Garrett, Alaine ; Ojeda-Torres, Geovannie ; ... More +
Coleman, Rebecca ; Lemire, Sharon W. ; Bragg, William ; Garrett, Alaine ; Ojeda-Torres, Geovannie ; Hamelin, Elizabeth ; Johnson, Rudolph C. ; Thomas, Jerry Less -
Description:
Tetrodotoxin (TTX) is an extremely potent paralytic toxin responsible for yearly illness and death around the world. A clinical measurement is necessary to confirm exposure because symptoms of TTX intoxication cannot be distinguished from other paralytic toxins. Our group has developed an online solid phase extraction hydrophilic interaction liquid chromatography (HILIC) method for the analysis of TTX in human urine with tandem mass spectrometry. The reportable range for the method was 2.80 - 249 ng/mL in urine with precision and accuracy within 15% as determined for all quality control samples. No isotopically-labeled internal standard is available for TTX; thus a surrogate internal standard, voglibose, was investigated to compensate for matrix effects and ionization suppression. However, upon evaluation, voglibose was ineffective for this purpose. This new online method rapidly identifies TTX, facilitating the work of public health authorities and providing support to monitoring programs worldwide.
Subjects:
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Article Calibration Chromatography, Liquid High-Throughput Screening Assays HILIC Humans Limit Of Detection Marine Toxins Online SPE Paralytic Reference Standards Solid Phase Extraction Tandem Mass Spectrometry Tetrodotoxin
Source:
Toxicon. 119:64-71
Pubmed ID:
27212629
Pubmed Central ID:
PMC6943750
Document Type:
Journal Article
Funding:
CC999999/ImCDC/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:ffb85754ceb99b8a5c4e5ee8dea10a6a00045338ecca19fa550ba92ba7e494e7
Download URL:
https://stacks.cdc.gov/view/cdc/84048/cdc_84048_DS1.pdf
File Type:

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