Distribution and Quantitative Estimates of Variant Creutzfeldt-Jakob Disease Prions in Tissues of Clinical and Asymptomatic Patients

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Douet, Jean Y. ; Lacroux, Caroline ; Aron, Naima ; Head, Mark W. ; Lugan, Séverine ; Tillier, Cécile ; Huor, Alvina ; Cassard, Hervé ; Arnold, Mark ; Beringue, Vincent ; Ironside, James W. ; Andréoletti, Olivier
• Description:
  In the United-Kingdom, ≈1 of 2,000 persons could be infected with variant Creutzfeldt-Jakob disease (vCJD). Therefore, risk of transmission of vCJD by medical procedures remains a major concern for public health authorities. In this study, we used in vitro amplification of prions by protein misfolding cyclic amplification (PMCA) to estimate distribution and level of the vCJD agent in 21 tissues from 4 patients who died of clinical vCJD and from 1 asymptomatic person with vCJD. PMCA identified major levels of vCJD prions in a range of tissues, including liver, salivary gland, kidney, lung, and bone marrow. Bioassays confirmed that the quantitative estimate of levels of vCJD prion accumulation provided by PMCA are indicative of vCJD infectivity levels in tissues. Findings provide critical data for the design of measures to minimize risk for iatrogenic transmission of vCJD.
• Subjects:
  Animals Asymptomatic Diseases Biological Assay Bone Marrow Creutzfeldt-Jakob Syndrome Female Humans Kidney Liver Lung Male Mice Protein Folding Prpc Proteins Salivary Glands

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• Source:
  Emerg Infect Dis. 23(6):946-956.
• Pubmed ID:
  28518033
• Pubmed Central ID:
  PMC5443438
• Document Type:
  Journal Article
• Place as Subject:
  United Kingdom
• Volume:
  23
• Issue:
  6
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:17d33055d91faea01ae53c1995884b0aff43738de5f6578d8e51f62340716c13
• Download URL:
  https://stacks.cdc.gov/view/cdc/45979/cdc_45979_DS1.pdf
• File Type:
  [PDF - 1.78 MB ]