Optimization of a Low Cost and Broadly Sensitive Genotyping Assay for HIV-1 Drug Resistance Surveillance and Monitoring in Resource-Limited Settings

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Zhou, Zhiyong ; Wagar, Nick ; DeVos, Joshua R. ; Rottinghaus, Erin ; Diallo, Karidia ; Nguyen, Duc B. ; Bassey, Orji ; Ugbena, Richard ; Wadonda-Kabondo, Nellie ; McConnell, Michelle S. ; Zulu, Isaac ; Chilima, Benson ; Nkengasong, John ; Yang, Chunfu
• Description:
  Conclusions
  
  The optimized in-house assay is broadly sensitive in genotyping HIV-1 group M viral strains and more sensitive than the original in-house, TRUGENE® and ViroSeq® in detecting mixed viral populations. The broad sensitivity and substantial reagent cost saving make this assay more accessible for RLS where HIVDR surveillance is recommended to minimize the development and transmission of HIVDR.
  
  
• Subjects:
  Amino Acids Base Sequence Biological Assay Costs And Cost Analysis Developing Countries Dried Blood Spot Testing Drug Resistance, Viral Genotyping Techniques Health Resources HIV-1 HIV Infections Humans Indicators And Reagents Molecular Sequence Data Mutation Population Surveillance Reproducibility Of Results Sequence Homology, Nucleic Acid

  More +
• Source:
  PLoS One. 2011; 6(11).
• Pubmed ID:
  22132237
• Pubmed Central ID:
  PMC3223235
• Document Type:
  Journal Article
• Volume:
  6
• Issue:
  11
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:864d7bfdc8d762af7c3a060c1d2e44d8641d64d5cbb99631453827cf0864e375
• Download URL:
  https://stacks.cdc.gov/view/cdc/4353/cdc_4353_DS1.pdf
• File Type:
  [PDF - 1.51 MB ]