Optimization of a Low Cost and Broadly Sensitive Genotyping Assay for HIV-1 Drug Resistance Surveillance and Monitoring in Resource-Limited Settings

Zhou, Zhiyong; Wagar, Nick; DeVos, Joshua R.; Rottinghaus, Erin; Diallo, Karidia; Nguyen, Duc B.; Bassey, Orji; Ugbena, Richard; Wadonda-Kabondo, Nellie; McConnell, Michelle S.; Zulu, Isaac; Chilima, Benson; Nkengasong, John; Yang, Chunfu

doi:10.1371/journal.pone.0028184

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Optimization of a Low Cost and Broadly Sensitive Genotyping Assay for HIV-1 Drug Resistance Surveillance and Monitoring in Resource-Limited Settings

Nov 23 2011
By Zhou, Zhiyong ; Wagar, Nick ; DeVos, Joshua R. ; ...
http://dx.doi.org/10.1371/journal.pone.0028184
Source: PLoS One. 2011; 6(11).

[PDF-1.51 MB]

English

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Details:

Alternative Title:

PLoS One
Personal Author:

Zhou, Zhiyong ; Wagar, Nick ; DeVos, Joshua R. ; Rottinghaus, Erin ; Diallo, Karidia ; ... More +

Zhou, Zhiyong ; Wagar, Nick ; DeVos, Joshua R. ; Rottinghaus, Erin ; Diallo, Karidia ; Nguyen, Duc B. ; Bassey, Orji ; Ugbena, Richard ; Wadonda-Kabondo, Nellie ; McConnell, Michelle S. ; Zulu, Isaac ; Chilima, Benson ; Nkengasong, John ; Yang, Chunfu Less -
Description:

Conclusions

The optimized in-house assay is broadly sensitive in genotyping HIV-1 group M viral strains and more sensitive than the original in-house, TRUGENE® and ViroSeq® in detecting mixed viral populations. The broad sensitivity and substantial reagent cost saving make this assay more accessible for RLS where HIVDR surveillance is recommended to minimize the development and transmission of HIVDR.
Subjects:

[+]

Amino Acids Base Sequence Biological Assay Costs And Cost Analysis Developing Countries Dried Blood Spot Testing Drug Resistance, Viral Genotyping Techniques Health Resources HIV-1 HIV Infections Humans Indicators And Reagents Molecular Sequence Data Mutation Population Surveillance Reproducibility Of Results Sequence Homology, Nucleic Acid
Source:

PLoS One. 2011; 6(11).
Pubmed ID:

22132237
Pubmed Central ID:

PMC3223235
Document Type:

Journal Article
Volume:

6
Issue:

11
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:864d7bfdc8d762af7c3a060c1d2e44d8641d64d5cbb99631453827cf0864e375
Download URL:

https://stacks.cdc.gov/view/cdc/4353/cdc_4353_DS1.pdf
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