Details:

Alternative Title:
PLoS One
Publisher's site:
http://dx.doi.org/
Personal Author:
Rottinghaus, Erin K. ; Beard, R. Suzanne ; Bile, Ebi ; Modukanele, Mosetsanagape ; Maruping, Maruping ; ... More +
Rottinghaus, Erin K. ; Beard, R. Suzanne ; Bile, Ebi ; Modukanele, Mosetsanagape ; Maruping, Maruping ; Mine, Madisa ; Nkengasong, John ; Yang, Chunfu Less -
Description:
As more HIV-infected people gain access to antiretroviral therapy (ART), monitoring HIV drug resistance (HIVDR) becomes essential to combat both acquired and transmitted HIVDR. Studies have demonstrated dried blood spots (DBS) are a suitable alternative in HIVDR monitoring using DBS collected on Whatman 903 (W-903). In this study, we sought to evaluate two other commercially available filter papers, Ahlstrom 226 (A-226) and Munktell TFN (M-TFN), for HIVDR genotyping following ambient temperature storage. DBS were prepared from remnant blood specimens collected from 334 ART patients and stored at ambient temperature for a median time of 30 days. HIV-1 viral load was determined using NucliSENS EasyQ® HIV-1 v2.0 RUO test kits prior to genotyping of the protease and reverse transcriptase regions of the HIV-1 pol gene using an in-house assay. Among the DBS tested, 26 specimens had a viral load ≥ 1000 copies/mL in all three types of filter paper and were included in the genotyping analysis. Genotyping efficiencies were similar between DBS collected on W-903 (92.3%), A-226 (88.5%), and M-TFN (92.3%) filter papers (P = 1.00). We identified 50 DR-associated mutations in DBS collected on W-903, 33 in DBS collected on A-226, and 48 in DBS collected on M-TFN, resulting in mutation detection sensitivities of 66.0% for A-226 and 88.0% for M-TFN when compared to W-903. Our data indicate that differences among filter papers may exist at this storage condition and warrant further studies evaluating filter paper type for HIVDR monitoring.
Subject:
[+]
Dried Blood Spot Testing
Drug Resistance, Viral
Filtration
Genotype
HIV-1
HIV Infections
Humans
Molecular Sequence Data
Mutation
Temperature
Viral Load

Source:
PLoS One. 2014; 9(10).
Series:
PEPFAR
Pubmed ID:
25303690
Pubmed Central ID:
PMC4193826
Document Type:
Journal Article
Funding:
PEPFAR/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:fd32546fe4dc3a9b3c5793e5d174e4457e57f3db205558d0437878b902dc2fcd
File Type:

Supporting Files

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