Details:

Alternative Title:
Gene
Personal Author:
Liu, Yao-Zhong ; Zhang, Lei ; Roy-Engel, Astrid M ; Saito, Shigeki ; Lasky, Joseph A ; ... More +
Liu, Yao-Zhong ; Zhang, Lei ; Roy-Engel, Astrid M ; Saito, Shigeki ; Lasky, Joseph A ; Wang, Guangdi ; Wang, He ; Less -
Description:
The health impacts of the BP oil spill are yet to be further revealed as the toxicological effects of oil products and dispersants on human respiratory system may be latent and complex, and hence difficult to study and follow up. Here we performed RNA-seq analyses of a system of human airway epithelial cells treated with the BP crude oil and/or dispersants Corexit 9500 and Corexit 9527 that were used to help break up the oil spill. Based on the RNA-seq data, we then systemically analyzed the transcriptomic perturbations of the cells at the KEGG pathway level using two pathway-based analysis tools, GAGE (generally applicable gene set enrichment) and GSNCA (Gene Sets Net Correlations Analysis). Our results suggested a pattern of change towards carcinogenesis for the treated cells marked by upregulation of ribosomal biosynthesis (hsa03008) (p=1.97E-13), protein processing (hsa04141) (p=4.09E-7), Wnt signaling (hsa04310) (p=6.76E-3), neurotrophin signaling (hsa04722) (p=7.73E-3) and insulin signaling (hsa04910) (p=1.16E-2) pathways under the dispersant Corexit 9527 treatment, as identified by GAGE analysis. Furthermore, through GSNCA analysis, we identified gene co-expression changes for several KEGG cancer pathways, including small cell lung cancer pathway (hsa05222, p=9.99E-5), under various treatments of oil/dispersant, especially the mixture of oil and Corexit 9527. Overall, our results suggested carcinogenic effects of dispersants (in particular Corexit 9527) and their mixtures with the BP crude oil, and provided further support for more stringent safety precautions and regulations for operations involving long-term respiratory exposure to oil and dispersants.
Subject:
[+]
Airway Epithelial Cells
Article
BP Oil Spill
Carcinogenesis
Carcinogens
Cell Transformation, Neoplastic
Dispersants
Epithelial Cells
GAGE
Gene Expression
GSNCA
Humans
KEGG
Lipids
Metabolic Networks And Pathways
Oil
Petroleum
Petroleum Pollution
Respiratory Mucosa
RNA-seq
Sequence Analysis, RNA
Signal Transduction
Surface-Active Agents
Water Pollutants, Chemical

Source:
Gene. 602:16-23. ;
Pubmed ID:
27866042
Pubmed Central ID:
PMC5191957
Document Type:
Journal Article ;
Funding:
G12 MD007595/MD/NIMHD NIH HHS/United States ; P20 GM103518/GM/NIGMS NIH HHS/United States ; P20 RR020152/RR/NCRR NIH HHS/United States ; T42 OH008421/OH/NIOSH CDC HHS/United States ;
G12 MD007595/MD/NIMHD NIH HHS/United States ; P20 GM103518/GM/NIGMS NIH HHS/United States ; P20 RR020152/RR/NCRR NIH HHS/United States ; T42 OH008421/OH/NIOSH CDC HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:133c873c75f201714b59f2071ef0ee2ae5106bff50801a2f49180f4a59975d1b
File Type:

Supporting Files

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