Neoplastic-like transformation effect of single-walled and multi-walled carbon nanotubes compared to asbestos on human lung small airway epithelial cells

Details

• Alternative Title:
  Nanotoxicology
• Personal Author:
  Wang, Liying ; Stueckle, Todd A. ; Mishra, Anurag ; Derk, Raymond ; Meighan, Terence ; Castranova, Vincent ; Rojanasakul, Yon
• Description:
  Accumulating evidence indicates that carbon nanotubes (CNTs) are biopersistent and can cause lung damage. With similar fibrous morphology and mode of exposure to asbestos, a known human carcinogen, growing concern has arisen for elevated risk of CNT-induced lung carcinogenesis; however, relatively little is known about the long-term carcinogenic effect of CNT. Neoplastic transformation is a key early event leading to carcinogenesis. We studied the ability of single- and multi-walled CNTs to induce neoplastic transformation of human lung epithelial cells compared to asbestos. Long-term (6-month) exposure of the cells to occupationally relevant concentrations of CNT in culture caused a neoplastic-like transformation phenotype as demonstrated by increased cell proliferation, anchorage-independent growth, invasion and angiogenesis. Whole-genome expression signature and protein expression analyses showed that single- and multi-walled CNTs shared similar signaling signatures which were distinct from asbestos. These results provide novel toxicogenomic information and suggest distinct particle-associated mechanisms of neoplasia promotion induced by CNTs and asbestos.
• Subjects:
  Asbestos Cell Line Cell Transformation, Neoplastic Epithelial Cells Gene Expression Profiling Humans Nanotubes, Carbon Proteins Respiratory Mucosa Signal Transduction
• Keywords:
  Genome Expression Invasion Lung Microarray Neoplastic Transformation
• Source:
  Nanotoxicology. 8(5):485-507
• Pubmed ID:
  23634900
• Pubmed Central ID:
  PMC4599578
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/ ; R01 HL095579/HL/NHLBI NIH HHSUnited States/ ; R01-HL095579/HL/NHLBI NIH HHSUnited States/
• Volume:
  8
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:dd6e072ffad3fcdbae5b05db961af68c9f72f606c6d0cb297d2582028c32c0300a00c6957f566304495a3de5cd7635dafc13daa76ee0ba9639ab944e3a1711f8
• Download URL:
  https://stacks.cdc.gov/view/cdc/34868/cdc_34868_DS1.pdf
• File Type:
  [PDF - 990.61 KB ]