Details:

Alternative Title:
PLoS One
Personal Author:
Griffing, Sean M. ; Mixson-Hayden, Tonya ; Sridaran, Sankar ; Alam, Md Tauqeer ; McCollum, Andrea M. ; ... More +
Griffing, Sean M. ; Mixson-Hayden, Tonya ; Sridaran, Sankar ; Alam, Md Tauqeer ; McCollum, Andrea M. ; Cabezas, César ; Marquiño Quezada, Wilmer ; Barnwell, John W. ; Macedo De Oliveira, Alexandre ; Lucas, Carmen ; Arrospide, Nancy ; Escalante, Ananias A. ; Bacon, David J. ; Udhayakumar, Venkatachalam Less -
Description:
Malaria has reemerged in many regions where once it was nearly eliminated. Yet the source of these parasites, the process of repopulation, their population structure, and dynamics are ill defined. Peru was one of malaria eradication's successes, where Plasmodium falciparum was nearly eliminated for two decades. It reemerged in the 1990s. In the new era of malaria elimination, Peruvian P. falciparum is a model of malaria reinvasion. We investigated its population structure and drug resistance profiles. We hypothesized that only populations adapted to local ecological niches could expand and repopulate and originated as vestigial populations or recent introductions. We investigated the genetic structure (using microsatellites) and drug resistant genotypes of 220 parasites collected from patients immediately after peak epidemic expansion (1999-2000) from seven sites across the country. The majority of parasites could be grouped into five clonal lineages by networks and AMOVA. The distribution of clonal lineages and their drug sensitivity profiles suggested geographic structure. In 2001, artesunate combination therapy was introduced in Peru. We tested 62 parasites collected in 2006-2007 for changes in genetic structure. Clonal lineages had recombined under selection for the fittest parasites. Our findings illustrate that local adaptations in the post-eradication era have contributed to clonal lineage expansion. Within the shifting confluence of drug policy and malaria incidence, populations continue to evolve through genetic outcrossing influenced by antimalarial selection pressure. Understanding the population substructure of P. falciparum has implications for vaccine, drug, and epidemiologic studies, including monitoring malaria during and after the elimination phase.
Subjects:
[+]
Antimalarials Dihydropteroate Synthase Drug Resistance Epidemics Gene Frequency Genotype Geography Haplotypes Humans Linkage Disequilibrium Malaria, Falciparum Membrane Transport Proteins Microsatellite Repeats Multidrug Resistance-Associated Proteins Phylogeny Plasmodium Falciparum Population Growth Protozoan Proteins Tetrahydrofolate Dehydrogenase
Source:
PLoS One. 2011; 6(9).
Document Type:
Journal Article
Place as Subject:
Peru
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:8cb446758308baf2e2a02858ef02671bc57670ab75a493db66bdc82658897d21
Download URL:
https://stacks.cdc.gov/view/cdc/4339/cdc_4339_DS1.pdf
File Type:

Supporting Files

• 	pone.0023486.g004.jpg	jpeg
  	pone.0023486.g005.gif	gif
  	pone.0023486.g005.jpg	jpeg
  	pone.0023486.g006.gif	gif
  	pone.0023486.g006.jpg	jpeg
  	pone.0023486.g007.gif	gif
  	pone.0023486.g007.jpg	jpeg
  	pone.0023486.nxml	txt
  	pone.0023486.t001.gif	gif
  	pone.0023486.t001.jpg	jpeg
  	license.txt	txt
  	pone.0023486.t002.gif	gif
  	pone.0023486.t002.jpg	jpeg
  	pone.0023486.t003.gif	gif
  	pone.0023486.t003.jpg	jpeg
  	pone.0023486.g001.gif	gif
  	pone.0023486.g001.jpg	jpeg
  	pone.0023486.g002.gif	gif
  	pone.0023486.g002.jpg	jpeg
  	pone.0023486.g003.gif	gif
  	pone.0023486.g003.jpg	jpeg
  	pone.0023486.g004.gif	gif

More +