Membrane Potential Dynamics of CA1 Pyramidal Neurons During Hippocampal Ripples in Awake Mice

Details

• Alternative Title:
  Neuron
• Personal Author:
  Hulse, Brad K. ; Moreaux, Laurent C. ; Lubenov, Evgueniy V. ; Siapas, Athanassios G.
• Description:
  Ripples are high-frequency oscillations associated with population bursts in area CA1 of the hippocampus that play a prominent role in theories of memory consolidation. While spiking during ripples has been extensively studied, our understanding of the subthreshold behavior of hippocampal neurons during these events remains incomplete. Here, we combine in vivo whole-cell and multisite extracellular recordings to characterize the membrane potential dynamics of identified CA1 pyramidal neurons during ripples. We find that the subthreshold depolarization during ripples is uncorrelated with the net excitatory input to CA1, while the post-ripple hyperpolarization varies proportionately. This clarifies the circuit mechanism keeping most neurons silent during ripples. On a finer timescale, the phase delay between intracellular and extracellular ripple oscillations varies systematically with membrane potential. Such smoothly varying delays are inconsistent with models of intracellular ripple generation involving perisomatic inhibition alone. Instead, they suggest that ripple-frequency excitation leading inhibition shapes intracellular ripple oscillations.
• Subjects:
  Animals Biological Clocks CA1 Region, Hippocampal Calbindins Lysine Male Membrane Potentials Mice Mice, Inbred C57BL Models, Neurological Nerve Net Nonlinear Dynamics Parvalbumins Patch-Clamp Techniques Pyramidal Cells Wakefulness

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• Source:
  Neuron. 89(4):800-813.
• Pubmed ID:
  26889811
• Pubmed Central ID:
  PMC5167572
• Document Type:
  Journal Article
• Funding:
  DP1 MH099907/MH/NIMH NIH HHS/United States ; DP1 OD008255/OD/NIH HHS/United States ; 5DP1MH099907/DP/NCCDPHP CDC HHS/United States ; NIH 1DP1OD008255/OD/NIH HHS/United States
• Volume:
  89
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:7979f75a544ddb1dc222f08f08e95ad767b22cb27dce951de8c4136fdc58bf9c
• Download URL:
  https://stacks.cdc.gov/view/cdc/43294/cdc_43294_DS1.pdf
• File Type:
  [PDF - 2.75 MB ]