Details:

Alternative Title:
ACS Appl Mater Interfaces
Publisher's site:
http://dx.doi.org/10.1021/acsami.5b10036
Personal Author:
Chattaraj, Rajarshi; Mohan, Praveena; Livingston, Clare M.; Besmer, Jeremy D.; Kumar, Kaushlendra; ... More +
Chattaraj, Rajarshi; Mohan, Praveena; Livingston, Clare M.; Besmer, Jeremy D.; Kumar, Kaushlendra; Goodwin, Andrew P.; Less -
Description:
Mutually reactive, fluorogenic molecules are presented as a simple and novel technique for in-solution biosensing. The hypothesis behind this work was that aggregating droplets into close proximity would cause rapid mixing of their contents. To take advantage of this effect, a novel pair of fluorogenic redox molecules were designed to remain in lipid-stabilized oil droplets but mix once aggregated. First, the hydrophobic cyanine dye 1,1'-dioctadecyl-3,3,3'3'-tetramethylindocarbocyanine perchlorate (DiI) was reduced with sodium borohydride to form a nonfluorescent analog (HDiI). Hydrophobic quinone derivatives were then screened as oxidizing agents, and it was found that p-fluoranil oxidized nonfluorescent HDiI back to fluorescent DiI. Next, HDiI and p-fluoranil were loaded into NEOBEE oil nanodroplets of average diameter 600 nm that were stabilized by a monolayer of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE)-polyethylene glycol (PEG), and DSPE-PEG-biotin. Addition of streptavidin caused aggregation of droplets and the appearance of red fluorescent aggregates within 30 min. Next, Nanoparticle Tracking Analysis was used to record the fluorescence of the droplets and their aggregates. By integrating the fluorescence emission of the tracked droplets, streptavidin could be detected down to 100 fM. Finally, the droplets were reformulated to sense for vascular endothelial growth factor (VEGF), a biomarker for tumor metastasis. Using anti-VEGF aptamers attached to DSPE-PEG incorporated into the nanodroplet monolayer, VEGF could also be detected down to 100 fM.
Subject:
[+]
Aptamers, Nucleotide
Article
Biomedical Applications
Biosensing Techniques
Biotinylation
Colloids
Fluorescent Dyes
Microscopy, Fluorescence
Nanoparticles
Protein Binding
Quinones
Self-Assembly
Sensors/Biosensors
Solutions
Spectrometry, Fluorescence
Stimuli-Responsive Materials
Streptavidin
Time Factors
Vascular Endothelial Growth Factor
Vascular Endothelial Growth Factor A

Source:
ACS Appl Mater Interfaces. 8(1):802-808.;
Pubmed ID:
26710239
Pubmed Central ID:
PMC5051691
Document Type:
Journal Article;
Funding:
R00 CA153935/CA/NCI NIH HHS/United States; S10 RR026641/RR/NCRR NIH HHS/United States; R21 EB018034/EB/NIBIB NIH HHS/United States; DP2EB020401/DP/NCCDPHP CDC HHS/United States; DP2 EB020401/EB/NIBIB NIH HHS/United States; ... More +
R00 CA153935/CA/NCI NIH HHS/United States; S10 RR026641/RR/NCRR NIH HHS/United States; R21 EB018034/EB/NIBIB NIH HHS/United States; DP2EB020401/DP/NCCDPHP CDC HHS/United States; DP2 EB020401/EB/NIBIB NIH HHS/United States; R00CA153935/CA/NCI NIH HHS/United States; S10RR026641/RR/NCRR NIH HHS/United States; R21EB018034/EB/NIBIB NIH HHS/United States; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:461fd3103ff9efe12202ca34c8b5593eb926768733da3e1f26bb1e38b8e16685
File Type:

Supporting Files

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