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CRISPR RNA-guided activation of endogenous human genes
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Jul 25 2013
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Source: Nat Methods. 10(10):977-979.
[PDF-206.72 KB]
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Alternative Title:Nat Methods
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Description:Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease (dCas9) to repress endogenous genes in bacteria and human cells. Here we show that single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain to increase expression of endogenous human genes. This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.
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Pubmed ID:23892898
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Pubmed Central ID:PMC3794058
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Volume:10
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Issue:10
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