CRISPR RNA-guided activation of endogenous human genes

Details

• Alternative Title:
  Nat Methods
• Personal Author:
  Maeder, Morgan L ; Linder, Samantha J ; Cascio, Vincent M ; Fu, Yanfang ; Ho, Quan H ; Joung, J Keith
• Description:
  Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease (dCas9) to repress endogenous genes in bacteria and human cells. Here we show that single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain to increase expression of endogenous human genes. This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.
• Subjects:
  Article Bacterial Proteins Clustered Regularly Interspaced Short Palindromic Repeats HEK293 Cells Humans Recombinant Fusion Proteins Ribonucleases RNA, Guide RNA Editing Streptococcus Pyogenes Transcriptional Activation Vascular Endothelial Growth Factor A

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• Source:
  Nat Methods. 10(10):977-979.
• Pubmed ID:
  23892898
• Pubmed Central ID:
  PMC3794058
• Document Type:
  Journal Article
• Funding:
  DP1 GM105378/DP/NCCDPHP CDC HHS/United States ; DP1 GM105378/GM/NIGMS NIH HHS/United States ; P50 HG005550/HG/NHGRI NIH HHS/United States ; R01 NS073124/NS/NINDS NIH HHS/United States
• Volume:
  10
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:74f61fb69e51a8c65eea41396a56b66a454137469d60a616d622aad9eeef791d
• Download URL:
  https://stacks.cdc.gov/view/cdc/22527/cdc_22527_DS1.pdf
• File Type:
  [PDF - 206.72 KB ]