Details:

Alternative Title:
Nat Methods
Personal Author:
Maeder, Morgan L ; Linder, Samantha J ; Cascio, Vincent M ; Fu, Yanfang ; Ho, Quan H ; ... More +
Maeder, Morgan L ; Linder, Samantha J ; Cascio, Vincent M ; Fu, Yanfang ; Ho, Quan H ; Joung, J Keith Less -
Description:
Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease (dCas9) to repress endogenous genes in bacteria and human cells. Here we show that single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain to increase expression of endogenous human genes. This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.
Subjects:
[+]
Article Bacterial Proteins Clustered Regularly Interspaced Short Palindromic Repeats HEK293 Cells Humans Recombinant Fusion Proteins Ribonucleases RNA, Guide RNA Editing Streptococcus Pyogenes Transcriptional Activation Vascular Endothelial Growth Factor A
Source:
Nat Methods. 10(10):977-979.
Pubmed ID:
23892898
Pubmed Central ID:
PMC3794058
Document Type:
Journal Article
Funding:
DP1 GM105378/DP/NCCDPHP CDC HHS/United States ; DP1 GM105378/GM/NIGMS NIH HHS/United States ; P50 HG005550/HG/NHGRI NIH HHS/United States ; P50 HG005550/HG/NHGRI NIH HHS/United States ; R01 NS073124/NS/NINDS NIH HHS/United States ; ... More +
DP1 GM105378/DP/NCCDPHP CDC HHS/United States ; DP1 GM105378/GM/NIGMS NIH HHS/United States ; P50 HG005550/HG/NHGRI NIH HHS/United States ; P50 HG005550/HG/NHGRI NIH HHS/United States ; R01 NS073124/NS/NINDS NIH HHS/United States ; R01 NS073124/NS/NINDS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:74f61fb69e51a8c65eea41396a56b66a454137469d60a616d622aad9eeef791d
Download URL:
https://stacks.cdc.gov/view/cdc/22527/cdc_22527_DS1.pdf
File Type:

Supporting Files

• 	NIHMS508103-supplement-1.pdf	pdf
  	NIHMS508103-supplement-2.xlsx	xlsx
  	nihms508103.nxml	bin
  	nihms508103f1.gif	gif
  	nihms508103f1.jpg	jpeg
  	nihms508103f2.gif	gif
  	nihms508103f2.jpg	jpeg

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