CRISPR RNA-guided activation of endogenous human genes

Maeder, Morgan L; Linder, Samantha J; Cascio, Vincent M; Fu, Yanfang; Ho, Quan H; Joung, J Keith

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CRISPR RNA-guided activation of endogenous human genes

Published Date:

Jul 25 2013

Source:

Nat Methods. 10(10):977-979.

[PDF - 206.72 KB]

Details:

Personal Authors:

Maeder, Morgan L ; Linder, Samantha J ; Cascio, Vincent M ; Fu, Yanfang ; Ho, Quan H ; ... More ▼

Keywords:

[+]

Pubmed ID:

23892898

Pubmed Central ID:

PMC3794058

Description:

Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease (dCas9) to repress endogenous genes in bacteria and human cells. Here we show that single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain to increase expression of endogenous human genes. This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

DP1 GM105378/DP/NCCDPHP CDC HHS/United States
DP1 GM105378/GM/NIGMS NIH HHS/United States
P50 HG005550/HG/NHGRI NIH HHS/United States
P50 HG005550/HG/NHGRI NIH HHS/United States
R01 NS073124/NS/NINDS NIH HHS/United States
R01 NS073124/NS/NINDS NIH HHS/United States

Supporting Files:

NIHMS508103-supplement-1.pdf	application/pdf
NIHMS508103-supplement-2.xlsx	application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
nihms508103.nxml	application/octet-stream
nihms508103f1.gif	image/gif
nihms508103f1.jpg	image/jpeg
nihms508103f2.gif	image/gif
nihms508103f2.jpg	image/jpeg

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