i
CRISPR RNA-guided activation of endogenous human genes
-
Jul 25 2013
Source: Nat Methods. 10(10):977-979.
Details:
-
Alternative Title:Nat Methods
-
Personal Author:
-
Description:Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease (dCas9) to repress endogenous genes in bacteria and human cells. Here we show that single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain to increase expression of endogenous human genes. This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.
-
Subjects:
-
Source:
-
Pubmed ID:23892898
-
Pubmed Central ID:PMC3794058
-
Document Type:
-
Funding:
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
Supporting Files
-
pdf xlsx bin gif jpeg gif jpeg
More +