Details:

Alternative Title:
PLoS One
Publisher's site:
http://dx.doi.org/10.1371/journal.pone.0157949
Personal Author:
Bharti, Praveen Kumar ; Chandel, Himanshu Singh ; Ahmad, Amreen ; Krishna, Sri ; Udhayakumar, Venkatachalam ; ... More +
Bharti, Praveen Kumar ; Chandel, Himanshu Singh ; Ahmad, Amreen ; Krishna, Sri ; Udhayakumar, Venkatachalam ; Singh, Neeru ; Less -
Description:
Background Plasmodium falciparum encoded histidine rich protein (HRP2) based malaria rapid diagnostic tests (RDTs) are used in India. Deletion of pfhrp2 and pfhrp3 genes contributes to false negative test results, and large numbers of such deletions have been reported from South America, highlighting the importance of surveillance to detect such deletions. Methods This is the first prospective field study carried out at 16 sites located in eight endemic states of India to assess the performance of PfHRP2 based RDT kits used in the national malaria control programme. In this study, microscopically confirmed P. falciparum but RDT negative samples were assessed for presence of pfhrp2, pfhrp3, and their flanking genes using PCR. Results Among 1521 microscopically positive P. falciparum samples screened, 50 were negative by HRP2 based RDT test. Molecular testing was carried out using these 50 RDT negative samples by assuming that 1471 RDT positive samples carried pfhrp2 gene. It was found that 2.4% (36/1521) and 1.8% (27/1521) of samples were negative for pfhrp2 and pfhrp3 genes, respectively. However, the frequency of pfhrp2 deletions varied between the sites ranging from 0–25% (2.4, 95% CI; 1.6–3.3). The frequency of both pfhrp2 and pfhrp3 gene deletion varied from 0–8% (1.6, 95% CI; 1.0–2.4). Conclusion This study provides evidence for low level presence of pfhrp2 and pfhrp3 deleted P. falciparum parasites in different endemic regions of India, and periodic surveillance is warranted for reliable use of PfHRP2 based RDTs.
Subject:
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Antigens, Protozoan
Diagnostic Tests, Routine
Endemic Diseases
Gene Deletion
Humans
Malaria, Falciparum
Plasmodium Falciparum
Prevalence
Prospective Studies
Protozoan Proteins

Source:
PLoS One. 11(8). ;
Pubmed ID:
27518538
Pubmed Central ID:
PMC4982695
Document Type:
Journal Article ;
Place as Subject:
India ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:8fa7a4cf5ed77f53b45bf751247005478fe4e5e198cb08119592ff2627edb604
File Type:

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