Prevalence of pfhrp2 and/or pfhrp3 Gene Deletion in Plasmodium falciparum Population in Eight Highly Endemic States in India

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Bharti, Praveen Kumar ; Chandel, Himanshu Singh ; Ahmad, Amreen ; Krishna, Sri ; Udhayakumar, Venkatachalam ; Singh, Neeru
• Description:
  Background
  
  Plasmodium falciparum encoded histidine rich protein (HRP2) based malaria rapid diagnostic tests (RDTs) are used in India. Deletion of pfhrp2 and pfhrp3 genes contributes to false negative test results, and large numbers of such deletions have been reported from South America, highlighting the importance of surveillance to detect such deletions.
  
  Methods
  
  This is the first prospective field study carried out at 16 sites located in eight endemic states of India to assess the performance of PfHRP2 based RDT kits used in the national malaria control programme. In this study, microscopically confirmed P. falciparum but RDT negative samples were assessed for presence of pfhrp2, pfhrp3, and their flanking genes using PCR.
  
  Results
  
  Among 1521 microscopically positive P. falciparum samples screened, 50 were negative by HRP2 based RDT test. Molecular testing was carried out using these 50 RDT negative samples by assuming that 1471 RDT positive samples carried pfhrp2 gene. It was found that 2.4% (36/1521) and 1.8% (27/1521) of samples were negative for pfhrp2 and pfhrp3 genes, respectively. However, the frequency of pfhrp2 deletions varied between the sites ranging from 0–25% (2.4, 95% CI; 1.6–3.3). The frequency of both pfhrp2 and pfhrp3 gene deletion varied from 0–8% (1.6, 95% CI; 1.0–2.4).
  
  Conclusion
  
  This study provides evidence for low level presence of pfhrp2 and pfhrp3 deleted P. falciparum parasites in different endemic regions of India, and periodic surveillance is warranted for reliable use of PfHRP2 based RDTs.
  
  
• Subjects:
  Antigens, Protozoan Diagnostic Tests, Routine Endemic Diseases Gene Deletion Humans Malaria, Falciparum Plasmodium Falciparum Prevalence Prospective Studies Protozoan Proteins
• Source:
  PLoS One. 11(8).
• Pubmed ID:
  27518538
• Pubmed Central ID:
  PMC4982695
• Document Type:
  Journal Article
• Place as Subject:
  India
• Volume:
  11
• Issue:
  8
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:8fa7a4cf5ed77f53b45bf751247005478fe4e5e198cb08119592ff2627edb604
• Download URL:
  https://stacks.cdc.gov/view/cdc/41402/cdc_41402_DS1.pdf
• File Type:
  [PDF - 2.76 MB ]