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Prevalence of pfhrp2 and/or pfhrp3 Gene Deletion in Plasmodium falciparum Population in Eight Highly Endemic States in India
Filetype[PDF-2.76 MB]


Details:
  • Alternative Title:
    PLoS One
  • Personal Author:
  • Description:
    Background

    Plasmodium falciparum encoded histidine rich protein (HRP2) based malaria rapid diagnostic tests (RDTs) are used in India. Deletion of pfhrp2 and pfhrp3 genes contributes to false negative test results, and large numbers of such deletions have been reported from South America, highlighting the importance of surveillance to detect such deletions.

    Methods

    This is the first prospective field study carried out at 16 sites located in eight endemic states of India to assess the performance of PfHRP2 based RDT kits used in the national malaria control programme. In this study, microscopically confirmed P. falciparum but RDT negative samples were assessed for presence of pfhrp2, pfhrp3, and their flanking genes using PCR.

    Results

    Among 1521 microscopically positive P. falciparum samples screened, 50 were negative by HRP2 based RDT test. Molecular testing was carried out using these 50 RDT negative samples by assuming that 1471 RDT positive samples carried pfhrp2 gene. It was found that 2.4% (36/1521) and 1.8% (27/1521) of samples were negative for pfhrp2 and pfhrp3 genes, respectively. However, the frequency of pfhrp2 deletions varied between the sites ranging from 0–25% (2.4, 95% CI; 1.6–3.3). The frequency of both pfhrp2 and pfhrp3 gene deletion varied from 0–8% (1.6, 95% CI; 1.0–2.4).

    Conclusion

    This study provides evidence for low level presence of pfhrp2 and pfhrp3 deleted P. falciparum parasites in different endemic regions of India, and periodic surveillance is warranted for reliable use of PfHRP2 based RDTs.

  • Subject:
  • Pubmed ID:
    27518538
  • Pubmed Central ID:
    PMC4982695
  • Document Type:
    Journal Article
  • Collection(s):
    CDC Public Access
  • Main Document Checksum:
This document has replaced (most recent first):
  1. 1989 Sexually transmitted diseases treatment guidelines
    Corporate Authors:
    Center for Prevention Services (U.S.). Division of Sexually Transmitted Diseases.
    Published Date:
    September 1, 1989
    Source:
    MMWR. Morbidity and mortality weekly report. 1989; 38 Suppl 8:1-43.
    Series:
    MMWR. Morbidity and mortality weekly report ; v. 38, suppl. no. 8
    DHHS publication ; no. (CDC) 85-8017
    Description:
    These guidelines for the treatment of patients with sexually transmitted diseases (STD) were established after consultation with a group of outside experts and staff of CDC. These guidelines are based on available efficacy data, practical applicabili...
    File Type:
    Filetype[PDF - 37.68 MB]
  2. 1985 STD treatment guidelines
    Corporate Authors:
    Center for Prevention Services (U.S.). Division of Sexually Transmitted Diseases.
    Published Date:
    October 18, 1985
    Source:
    MMWR Suppl. 1985 Oct 18;34(4):75S-108S
    Series:
    MMWR. Morbidity and mortality weekly report ; v. 34, suppl. 4
    HHS publication ; no. (CDC) 85-8017
    Description:
    This document is the same as 1985 STD Treatment Guidelines (Atlanta: CDC, September 1985).These guidelines for treatment of sexually transmitted diseases (STD) were established after careful deliberation by a group of experts* and staff of the Center...
    Status:
    superseded
    File Type:
    Filetype[PDF - 28.36 MB]
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