The cremeomycin biosynthetic gene cluster encodes a pathway for diazo formation**

Details

• Alternative Title:
  Chembiochem
• Personal Author:
  Waldman, Abraham J. ; Pechersky, Yakov ; Wang, Peng ; Wang, Jennifer X. ; Balskus, Emily P.
• Description:
  Diazo groups are found in a range of natural products that possess potent biological activities. Despite longstanding interest in these metabolites, diazo group biosynthesis is not well understood, in part because of difficulties in identifying specific genes linked to diazo formation. Here we describe the discovery of the gene cluster that produces the o-diazoquinone natural product cremeomycin and its heterologous expression in Streptomyces lividans. We used stable isotope feeding experiments and in vitro characterization of biosynthetic enzymes to decipher the order of events in this pathway and establish that diazo construction involves late-stage N-N bond formation. This work represents the first successful production of a diazo-containing metabolite in a heterologous host, experimentally linking a set of genes with diazo formation.
• Subjects:
  • Azo Compounds
  • Biosynthetic Pathways
  • Cyclohexanones
  • Molecular Structure
  • Multigene Family
  • Streptomyces lividans
• Keywords:
  • natural products
  • biosynthesis
  • diazo compounds
  • enzymes
  • heterologous expression
• Source:
  Chembiochem. 16(15):2172-2175
• Pubmed ID:
  26278892
• Pubmed Central ID:
  PMC4996270
• Document Type:
  Journal Article
• Funding:
  DP2 GM105434/GM/NIGMS NIH HHSUnited States/ ; T32 GM095450/GM/NIGMS NIH HHSUnited States/ ; DP2 GM105434/DP/NCCDPHP CDC HHSUnited States/ ; GM095450/GM/NIGMS NIH HHSUnited States/
• Volume:
  16
• Issue:
  15
• Download URL:
  https://stacks.cdc.gov/view/cdc/41079/cdc_41079_DS1.pdf
• File Type:
  [PDF - 1.32 MB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:aec79476552a7f40cf40150624b5c9e827f065fc8efa7060a8cf3e4d9b54fbe9