Discovery of Cryptic Polyketide Metabolites from Dermatophytes using Heterologous Expression in Aspergillus nidulans

Details

• Alternative Title:
  ACS Synth Biol
• Personal Author:
  Yin, Wen-Bing ; Chooi, Yit Heng ; Smith, Adam R. ; Cacho, Ralph A. ; Hu, Youcai ; White, Theodore C. ; Tang, Yi
• Description:
  Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains.
• Subjects:
  Arthrodermataceae Article Aspergillus Nidulans Cloning, Molecular Fungal Proteins Genes, Fungal Heterologous Expression Multigene Family Mutation Natural Products Polyketide Polyketides Polyketide Synthases Prenyltransferase Secondary Metabolism

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• Source:
  ACS Synth Biol. 2(11):629-634
• Pubmed ID:
  23758576
• Pubmed Central ID:
  PMC3795930
• Document Type:
  Journal Article
• Funding:
  DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; R21 AI081235/AI/NIAID NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/ ; R21AI081235/AI/NIAID NIH HHSUnited States/
• Volume:
  2
• Issue:
  11
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f7b879b91493a7f9f9123664be12adb05c38e4eae677696b8298bb6292a28988
• Download URL:
  https://stacks.cdc.gov/view/cdc/29756/cdc_29756_DS1.pdf
• File Type:
  [PDF - 882.77 KB ]