Details:

Alternative Title:
ACS Synth Biol
Publisher's site:
http://dx.doi.org/10.1021/sb400048b
Personal Author:
Yin, Wen-Bing ; Chooi, Yit Heng ; Smith, Adam R. ; Cacho, Ralph A. ; Hu, Youcai ; ... More +
Yin, Wen-Bing ; Chooi, Yit Heng ; Smith, Adam R. ; Cacho, Ralph A. ; Hu, Youcai ; White, Theodore C. ; Tang, Yi Less -
Description:
Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains.
Subject:
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Arthrodermataceae
Article
Aspergillus Nidulans
Cloning, Molecular
Fungal Proteins
Genes, Fungal
Heterologous Expression
Multigene Family
Mutation
Natural Products
Polyketide
Polyketides
Polyketide Synthases
Prenyltransferase
Secondary Metabolism

Source:
ACS Synth Biol. 2(11):629-634.
Pubmed ID:
23758576
Pubmed Central ID:
PMC3795930
Document Type:
Journal Article
Funding:
1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; DP1 GM106413/GM/NIGMS NIH HHS/United States ; R21AI081235/AI/NIAID NIH HHS/United States
1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; DP1 GM106413/GM/NIGMS NIH HHS/United States ; R21AI081235/AI/NIAID NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:f7b879b91493a7f9f9123664be12adb05c38e4eae677696b8298bb6292a28988
File Type:

Supporting Files

• 	nihms-489653.nxml	txt
  	NIHMS489653-supplement-1_si_001.pdf	pdf
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