Combinatorial Generation of Chemical Diversity by Redox Enzymes in Chaetoviridin Biosynthesis

Details

• Alternative Title:
  Org Lett
• Personal Author:
  Sato, Michio ; Winter, Jaclyn M. ; Noguchi, Hiroshi ; Tang, Yi ; Watanabe, Kenji
• Description:
  Chaetoviridins constitute a large family of structurally related secondary metabolites isolated from Chaetomium fungi. To elucidate the biosynthesis pathway and understand how the chemical diversity of chaetoviridins is generated, gene deletion and in vitro characterization of the four post-PKS modifications enzymes were undertaken. CazL and CazP were identified to have substrate promiscuity that facilitates the formation of nonchlorinated analogues. In addition, enzymatic oxidation and reduction combined with spontaneous dehydration and lactonization of the intermediates further expand the chemical diversity.
• Subjects:
  Article Biosynthetic Pathways Chaetomium Furans Gene Deletion Molecular Structure Oxidation-Reduction Polyketide Synthases Pyrones
• Source:
  Org Lett. 18(6):1446-1449
• Pubmed ID:
  26959241
• Pubmed Central ID:
  PMC4903021
• Document Type:
  Journal Article
• Funding:
  DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/
• Volume:
  18
• Issue:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:134d7761a71e5da5968d4f1033b2957ec612ba73ecdac2e00101e5b8781bccce
• Download URL:
  https://stacks.cdc.gov/view/cdc/40284/cdc_40284_DS1.pdf
• File Type:
  [PDF - 643.58 KB ]