Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications

Details

• Alternative Title:
  Sci Rep
• Personal Author:
  Zhang, Yu Shrike ; Chang, Jae-Byum ; Alvarez, Mario Moisés ; Trujillo-de Santiago, Grissel ; Aleman, Julio ; Batzaya, Byambaa ; Krishnadoss, Vaishali ; Ramanujam, Aishwarya Aravamudhan ; Kazemzadeh-Narbat, Mehdi ; Chen, Fei ; Tillberg, Paul W. ; Dokmeci, Mehmet Remzi ; Boyden, Edward S. ; Khademhosseini, Ali
• Description:
  To date, much effort has been expended on making high-performance microscopes through better instrumentation. Recently, it was discovered that physical magnification of specimens was possible, through a technique called expansion microscopy (ExM), raising the question of whether physical magnification, coupled to inexpensive optics, could together match the performance of high-end optical equipment, at a tiny fraction of the price. Here we show that such "hybrid microscopy" methods--combining physical and optical magnifications--can indeed achieve high performance at low cost. By physically magnifying objects, then imaging them on cheap miniature fluorescence microscopes ("mini-microscopes"), it is possible to image at a resolution comparable to that previously attainable only with benchtop microscopes that present costs orders of magnitude higher. We believe that this unprecedented hybrid technology that combines expansion microscopy, based on physical magnification, and mini-microscopy, relying on conventional optics--a process we refer to as Expansion Mini-Microscopy (ExMM)--is a highly promising alternative method for performing cost-effective, high-resolution imaging of biological samples. With further advancement of the technology, we believe that ExMM will find widespread applications for high-resolution imaging particularly in research and healthcare scenarios in undeveloped countries or remote places.
• Subjects:
  Animals Brain Cost-Benefit Analysis HEK293 Cells Humans Luminescent Proteins Mice Mice, Inbred C57BL Mice, Transgenic Microscopy Microscopy, Confocal Microscopy, Fluorescence NIH 3T3 Cells Reproducibility Of Results Tubulin

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• Source:
  Sci Rep. 2016; 6.
• Pubmed ID:
  26975883
• Pubmed Central ID:
  PMC4792139
• Document Type:
  Journal Article
• Funding:
  1DP1NS087724/DP/NCCDPHP CDC HHS/United States ; 1R01MH103910/MH/NIMH NIH HHS/United States ; 1U01MH106011/MH/NIMH NIH HHS/United States ; AR057837/AR/NIAMS NIH HHS/United States ; DE021468/DE/NIDCR NIH HHS/United States ; DP1 NS087724/NS/NINDS NIH HHS/United States ; EB012597/EB/NIBIB NIH HHS/United States ; HL099073/HL/NHLBI NIH HHS/United States ; R56AI105024/AI/NIAID NIH HHS/United States ; U01 MH106011/MH/NIMH NIH HHS/United States
• Volume:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:6dd2514a8ae091d1906c5982380cbf8389b1ca860060677c2744d8f08b4132f0
• Download URL:
  https://stacks.cdc.gov/view/cdc/41049/cdc_41049_DS1.pdf
• File Type:
  [PDF - 1.69 MB ]