Simultaneous multi-plane imaging of neural circuits

Details

• Alternative Title:
  Neuron
• Personal Author:
  Yang, Weijian ; Miller, Jae-eun Kang ; Carrillo-Reid, Luis ; Pnevmatikakis, Eftychios ; Paninski, Liam ; Yuste, Rafael ; Peterka, Darcy S.
• Description:
  Recording the activity of large populations of neurons is an important step toward understanding the emergent function of neural circuits. Here we present a simple holographic method to simultaneously perform two-photon calcium imaging of neuronal populations across multiple areas and layers of mouse cortex in vivo. We use prior knowledge of neuronal locations, activity sparsity, and a constrained nonnegative matrix factorization algorithm to extract signals from neurons imaged simultaneously and located in different focal planes or fields of view. Our laser multiplexing approach is simple and fast, and could be used as a general method to image the activity of neural circuits in three dimensions across multiple areas in the brain.
• Subjects:
  Animals Article Calcium Cerebral Cortex Mice Mice, Inbred C57BL Microscopy, Fluorescence, Multiphoton Nerve Net Neurons
• Source:
  Neuron. 89(2):269-284
• Pubmed ID:
  26774159
• Pubmed Central ID:
  PMC4724224
• Document Type:
  Journal Article
• Funding:
  R01MH100561/MH/NIMH NIH HHS/United States ; R01MH101218/MH/NIMH NIH HHS/United States ; R01 MH100561/MH/NIMH NIH HHS/United States ; R01 MH101218/MH/NIMH NIH HHS/United States ; DP1 EY024503/EY/NEI NIH HHS/United States ; R01 EY011787/EY/NEI NIH HHS/United States ; R41MH100895/MH/NIMH NIH HHS/United States ; R41 MH100895/MH/NIMH NIH HHS/United States ; DP1EY024503/DP/NCCDPHP CDC HHS/United States ; R01EY011787/EY/NEI NIH HHS/United States
• Volume:
  89
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f16784bba612fa315688286aac5f6845c81cc7f0d53ef1f5fb623f7d3709215d
• Download URL:
  https://stacks.cdc.gov/view/cdc/39482/cdc_39482_DS1.pdf
• File Type:
  [PDF - 4.22 MB ]