Expansion Microscopy

Chen, Fei; Tillberg, Paul W.; Boyden, Edward S.

doi:10.1126/science.1260088

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Expansion Microscopy

Published Date:

Jan 15 2015

Publisher's site:

http://dx.doi.org/10.1126/science.1260088 New Site Icon

Source:

Science. 347(6221):543-548.

[PDF - 1013.63 KB]

Details:

Personal Authors:

Chen, Fei ; Tillberg, Paul W. ; Boyden, Edward S. ;

Keywords:

[+]

Pubmed ID:

25592419

Pubmed Central ID:

PMC4312537

Description:

In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal microscope.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

1DP1NS087724/DP/NCCDPHP CDC HHS/United States
1R01MH103910-01/MH/NIMH NIH HHS/United States
DP1 NS087724/NS/NINDS NIH HHS/United States
R01 MH103910/MH/NIMH NIH HHS/United States

Supporting Files:

NIHMS649590-supplement-Supplementary_Materials.docx	application/vnd.openxmlformats-officedocument.wordprocessingml.document
NIHMS649590-supplement-Supp_Movie_1.m4v	video/x-m4v
NIHMS649590-supplement-Supp_Movie_2.m4v	video/x-m4v
NIHMS649590-supplement-Supp_Movie_3.m4v	video/x-m4v
nihms649590.nxml	text/plain
nihms649590f1.gif	image/gif
nihms649590f1.jpg	image/jpeg
nihms649590f2.gif	image/gif
nihms649590f2.jpg	image/jpeg
nihms649590f3.gif	image/gif
nihms649590f3.jpg	image/jpeg
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nihms649590f4.jpg	image/jpeg

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