Expansion Microscopy

Chen, Fei; Tillberg, Paul W.; Boyden, Edward S.

doi:10.1126/science.1260088

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Expansion Microscopy

Published Date:

Jan 15 2015

Publisher's site:

http://dx.doi.org/10.1126/science.1260088 New Site Icon

Source:

Science. 347(6221):543-548.

[PDF-1013.63 KB]

Details:

Alternative Title:

Science

Personal Author:

Chen, Fei ; Tillberg, Paul W. ; Boyden, Edward S. ;

Description:

In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal microscope.

Subject:

[+]

Pubmed ID:

25592419

Pubmed Central ID:

PMC4312537

Document Type:

Journal Article

Funding:

1DP1NS087724/DP/NCCDPHP CDC HHS/United States ; 1R01MH103910-01/MH/NIMH NIH HHS/United States ; DP1 NS087724/NS/NINDS NIH HHS/United States ; R01 MH103910/MH/NIMH NIH HHS/United States ;

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

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