Expansion Microscopy

Details

• Alternative Title:
  Science
• Personal Author:
  Chen, Fei ; Tillberg, Paul W. ; Boyden, Edward S.
• Description:
  In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal microscope.
• Subjects:
  Acrylamide Acrylamides Acrylates Animals Article Coated Pits, Cell-Membrane Fluorescent Dyes Gels HEK293 Cells Hippocampus Humans Mice, Inbred C57BL Mice, Transgenic Microscopy Microscopy, Confocal Microscopy, Fluorescence Microtubules Optical Imaging Polymers Tissue Fixation

  More +
• Source:
  Science. 347(6221):543-548.
• Pubmed ID:
  25592419
• Pubmed Central ID:
  PMC4312537
• Document Type:
  Journal Article
• Funding:
  1DP1NS087724/DP/NCCDPHP CDC HHS/United States ; 1R01MH103910-01/MH/NIMH NIH HHS/United States ; DP1 NS087724/NS/NINDS NIH HHS/United States ; R01 MH103910/MH/NIMH NIH HHS/United States
• Volume:
  347
• Issue:
  6221
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:6e059be7ee19365a37efd59f9ce92a35338dbdb931af4438c5bfd543ec3226fd
• Download URL:
  https://stacks.cdc.gov/view/cdc/30274/cdc_30274_DS1.pdf
• File Type:
  [PDF - 1013.63 KB ]