Details:

Alternative Title:
Science
Publisher's site:
http://dx.doi.org/10.1126/science.1260088
Personal Author:
Chen, Fei ; Tillberg, Paul W. ; Boyden, Edward S. ;
Chen, Fei ; Tillberg, Paul W. ; Boyden, Edward S. ; Less -
Description:
In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~10(7) cubic micrometers of the mouse hippocampus with a conventional confocal microscope.
Subject:
[+]
Acrylamide
Acrylamides
Acrylates
Animals
Article
Coated Pits, Cell-Membrane
Fluorescent Dyes
Gels
HEK293 Cells
Hippocampus
Humans
Mice, Inbred C57BL
Mice, Transgenic
Microscopy
Microscopy, Confocal
Microscopy, Fluorescence
Microtubules
Optical Imaging
Polymers
Tissue Fixation

Source:
Science. 347(6221):543-548. ;
Pubmed ID:
25592419
Pubmed Central ID:
PMC4312537
Document Type:
Journal Article ;
Funding:
1DP1NS087724/DP/NCCDPHP CDC HHS/United States ; 1R01MH103910-01/MH/NIMH NIH HHS/United States ; DP1 NS087724/NS/NINDS NIH HHS/United States ; R01 MH103910/MH/NIMH NIH HHS/United States ;
1DP1NS087724/DP/NCCDPHP CDC HHS/United States ; 1R01MH103910-01/MH/NIMH NIH HHS/United States ; DP1 NS087724/NS/NINDS NIH HHS/United States ; R01 MH103910/MH/NIMH NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:6e059be7ee19365a37efd59f9ce92a35338dbdb931af4438c5bfd543ec3226fd
File Type:

Supporting Files

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