Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Kilianski, Andy ; Roth, Pierce A. ; Liem, Alvin T. ; Hill, Jessica M. ; Willis, Kristen L. ; Rossmaier, Rebecca D. ; Marinich, Andrew V. ; Maughan, Michele N. ; Karavis, Mark A. ; Kuhn, Jens H. ; Honko, Anna N. ; Rosenzweig, C. Nicole
• Description:
  Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization.
• Subjects:
  Dispatch Ebola Virus Fieldable Platform Genomics Nanopore Sequencing RNA Virus Sequencing Use Of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing For Rapid Detection And Characterization Of RNA Viruses Venezuelan Equine Encephalitis Virus Viruses Zika Virus

  More +
• Source:
  Emerg Infect Dis. 22(8):1448-1451.
• Pubmed ID:
  27191483
• Pubmed Central ID:
  PMC4982148
• Document Type:
  Journal Article
• Volume:
  22
• Issue:
  8
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:9837b449929ecbf8ae67334700a7d9bb1b954783efbfbe350139bec8dc082c8c
• Download URL:
  https://stacks.cdc.gov/view/cdc/40872/cdc_40872_DS1.pdf
• File Type:
  [PDF - 825.52 KB ]