Welcome to CDC stacks | A programmable DNA origami platform to organize SNAREs for membrane fusion - 40733 | CDC Public Access
Stacks Logo
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.
Clear All Simple Search
Advanced Search
A programmable DNA origami platform to organize SNAREs for membrane fusion
Filetype[PDF-1.07 MB]

  • Pubmed ID:
  • Pubmed Central ID:
  • Description:
    Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes are the core molecular machinery of membrane fusion, a fundamental process that drives inter- and intracellular communication and trafficking. One of the questions that remains controversial has been whether and how SNAREs cooperate. Here we show the use of self-assembled DNA-nanostructure rings to template uniform-sized small unilamellar vesicles containing predetermined maximal number of externally facing SNAREs to study the membrane-fusion process. We also incorporated lipid-conjugated complementary ssDNA as tethers into vesicle and target membranes, which enabled bypass of the rate-limiting docking step of fusion reactions and allowed direct observation of individual membrane-fusion events at SNARE densities as low as one pair per vesicle. With this platform, we confirmed at the single event level that, after docking of the templated-SUVs to supported lipid bilayers (SBL), one to two pairs of SNAREs are sufficient to drive fast lipid mixing. Modularity and programmability of this platform makes it readily amenable to studying more complicated systems where auxiliary proteins are involved.

  • Document Type:
  • Collection(s):
  • Funding:
    DP2-OD004641/OD/NIH HHS/United States
    R01 DK027044/DK/NIDDK NIH HHS/United States
    DP2 GM114830/GM/NIGMS NIH HHS/United States
    DP2 OD004641/OD/NIH HHS/United States
    R37 DK027044/DK/NIDDK NIH HHS/United States
    DK027044-39/DK/NIDDK NIH HHS/United States
    DP2-GM114830/DP/NCCDPHP CDC HHS/United States
    R21 GM109466/GM/NIGMS NIH HHS/United States
    R01 GM108954/GM/NIGMS NIH HHS/United States
    R01GM108954/GM/NIGMS NIH HHS/United States
    R21-GM109466/GM/NIGMS NIH HHS/United States
No Related Documents.
You May Also Like: