Details:

Alternative Title:
Nat Struct Mol Biol
Personal Author:
Heider, Margaret R. ; Gu, Mingyu ; Duffy, Caroline M. ; Mirza, Anne M. ; Marcotte, Laura L. ; ... More +
Heider, Margaret R. ; Gu, Mingyu ; Duffy, Caroline M. ; Mirza, Anne M. ; Marcotte, Laura L. ; Walls, Alexandra C. ; Farrall, Nicholas ; Hakhverdyan, Zhanna ; Field, Mark C. ; Rout, Michael P. ; Frost, Adam ; Munson, Mary Less -
Description:
The exocyst is a hetero-octameric complex that has been proposed to serve as the tethering complex for exocytosis, although it remains poorly understood at the molecular level. Here, we purified endogenous exocyst complexes from Saccharomyces cerevisiae and showed that they are stable and consist of all eight subunits with equal stoichiometry. Using a combination of biochemical and auxin induced-degradation experiments in yeast, we mapped the subunit connectivity, identified two stable four-subunit modules within the octamer and demonstrated that several known exocyst-binding partners are not necessary for exocyst assembly and stability. Furthermore, we visualized the structure of the yeast complex by using negative-stain electron microscopy; our results indicate that the exocyst exists predominantly as a stable, octameric complex with an elongated architecture that suggests that the subunits are contiguous helical bundles packed together into a bundle of long rods.
Subject:
[+]
Article
Exocytosis
Macromolecular Substances
Microscopy, Electron, Transmission
Protein Binding
Protein Stability
Protein Structure, Quaternary
Saccharomyces Cerevisiae
Vesicular Transport Proteins

Source:
Nat Struct Mol Biol. 23(1):59-66.
Pubmed ID:
26656853
Pubmed Central ID:
PMC4752824
Document Type:
Journal Article
Funding:
1DP2GM110772/DP/NCCDPHP CDC HHS/United States ; DP2 GM110772/GM/NIGMS NIH HHS/United States ; GM068803/GM/NIGMS NIH HHS/United States ; P41 GM109824/GM/NIGMS NIH HHS/United States ; P41 GM109824/GM/NIGMS NIH HHS/United States ; ... More +
1DP2GM110772/DP/NCCDPHP CDC HHS/United States ; DP2 GM110772/GM/NIGMS NIH HHS/United States ; GM068803/GM/NIGMS NIH HHS/United States ; P41 GM109824/GM/NIGMS NIH HHS/United States ; P41 GM109824/GM/NIGMS NIH HHS/United States ; R01 GM068803/GM/NIGMS NIH HHS/United States ; U54 GM103511/GM/NIGMS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:5e03a068e91e8682010f253f33d550504c8e27f15cc27543cbfbae1352a84f96
File Type:

Supporting Files

• 	nihms758359f4.gif	gif
  	nihms758359f4.jpg	jpeg
  	nihms758359f5.gif	gif
  	nihms758359f5.jpg	jpeg
  	nihms758359f6.gif	gif
  	nihms758359f6.jpg	jpeg
  	NIHMS758359-supplement-Supplemental_figures.pdf	pdf
  	nihms758359.nxml	bin
  	nihms758359f1.gif	gif
  	nihms758359f1.jpg	jpeg
  	nihms758359f2.gif	gif
  	nihms758359f2.jpg	jpeg
  	nihms758359f3.gif	gif
  	nihms758359f3.jpg	jpeg

More +