Details:

Alternative Title:
Nat Commun
Personal Author:
Qiu, Yupeng ; Antony, Edwin ; Doganay, Sultan ; Ran Koh, Hye ; Lohman, Timothy ; ... More +
Qiu, Yupeng ; Antony, Edwin ; Doganay, Sultan ; Ran Koh, Hye ; Lohman, Timothy ; Myong, Sua Less -
Description:
Srs2 dismantles presynaptic Rad51 filaments and prevents its re-formation as an anti-recombinase. However, the molecular mechanism by which Srs2 accomplishes these tasks remains unclear. Here we report a single-molecule fluorescence study of the dynamics of Rad51 filament formation and its disruption by Srs2. Rad51 forms filaments on single-stranded DNA by sequential binding of primarily monomers and dimers in a 5'-3' direction. One Rad51 molecule binds to three nucleotides, and six monomers are required to achieve a stable nucleation cluster. Srs2 exhibits ATP-dependent repetitive motion on single-stranded DNA and this activity prevents re-formation of the Rad51 filament. The same activity of Srs2 cannot prevent RecA filament formation, indicating its specificity for Rad51. Srs2's DNA-unwinding activity is greatly suppressed when Rad51 filaments form on duplex DNA. Taken together, our results reveal an exquisite and highly specific mechanism by which Srs2 regulates the Rad51 filament formation.
Subjects:
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Adenosine Triphosphate Article DNA, Fungal DNA, Single-Stranded DNA-Binding Proteins DNA Helicases Homologous Recombination Protein Binding Rad51 Recombinase Rec A Recombinases Saccharomyces Cerevisiae Saccharomyces Cerevisiae Proteins
Source:
Nat Commun. 4:2281
Pubmed ID:
23939144
Pubmed Central ID:
PMC3979297
Document Type:
Journal Article
Funding:
DP2 GM105453/GM/NIGMS NIH HHS/United States ; R01 GM045948/GM/NIGMS NIH HHS/United States ; 1DP2GM105453/DP/NCCDPHP CDC HHS/United States ; GM045948/GM/NIGMS NIH HHS/United States
DP2 GM105453/GM/NIGMS NIH HHS/United States ; R01 GM045948/GM/NIGMS NIH HHS/United States ; 1DP2GM105453/DP/NCCDPHP CDC HHS/United States ; GM045948/GM/NIGMS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:53c8d42517e6d20a9974655e8902f9616063f1e67038618566b1e7cebee844cf
Download URL:
https://stacks.cdc.gov/view/cdc/29911/cdc_29911_DS1.pdf
File Type:

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