Optical coherence contrast imaging using gold nanorods in living mice eyes

Details

• Alternative Title:
  Clin Experiment Ophthalmol
• Personal Author:
  de la Zerda, Adam ; Prabhulkar, Shradha ; Perez, Victor L ; Ruggeri, Marco ; Paranjape, Amit S ; Habte, Frezghi ; Gambhir, Sanjiv S ; Awdeh, Richard M
• Description:
  Background
  
  Optical coherence tomography (OCT) is a powerful imaging modality to visualize tissue structures, with axial image pixel resolution as high as 1.6 μm in tissue. However, OCT is intrinsically limited to providing structural information as the OCT contrast is produced by optically scattering tissues.
  
  Methods
  
  Gold nanorods (GNRs) were injected into the anterior chamber (AC) and cornea of mice eyes which could create a significant OCT signal and hence could be used as a contrast agent for in vivo OCT imaging.
  
  Results
  
  A dose of 30 nM of GNRs (13 nm in diameter and 45 nm in length) were injected to the AC of mice eyes and produced an OCT contrast nearly 50-fold higher than control mice injected with saline. Furthermore, the lowest detectable concentration of GNRs in living mice AC was experimentally estimated to be as low as 120 pM.
  
  Conclusions
  
  The high sensitivity and low toxicity of GNRs brings great promise for OCT to uniquely become a high-resolution molecular imaging modality.
  
  
• Subjects:
  Anatomy, Cross-Sectional Animals Anterior Chamber Article Contrast Agent Contrast Media Cornea Gold Gold Nanorods Imaging, Three-Dimensional Mice Mice, Inbred C57BL Molecular Imaging Nanotubes Ophthalmic Imaging Optical Coherence Tomography Phantoms, Imaging Tomography, Optical Coherence

  More +
• Source:
  Clin Experiment Ophthalmol. 43(4):358-366
• Pubmed ID:
  24533647
• Pubmed Central ID:
  PMC4963150
• Document Type:
  Journal Article
• Funding:
  R21 EY020940/EY/NEI NIH HHS/United States ; R21EY020940/EY/NEI NIH HHS/United States ; DP5 0D012179/DP/NCCDPHP CDC HHS/United States ; U54 CA119367/CA/NCI NIH HHS/United States ; DP5 OD012179/OD/NIH HHS/United States ; P50 CA114747/CA/NCI NIH HHS/United States
• Volume:
  43
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:a03b7c039d0122ea10e529d30dd79ad0945786a4432aa1ad0d5985e2e1090f36
• Download URL:
  https://stacks.cdc.gov/view/cdc/40550/cdc_40550_DS1.pdf
• File Type:
  [PDF - 400.39 KB ]