Optical coherence contrast imaging using gold nanorods in living mice eyes

de la Zerda, Adam; Prabhulkar, Shradha; Perez, Victor L; Ruggeri, Marco; Paranjape, Amit S; Habte, Frezghi; Gambhir, Sanjiv S; Awdeh, Richard M

doi:10.1111/ceo.12299

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Optical coherence contrast imaging using gold nanorods in living mice eyes

Published Date:

2015 May-Jun

Publisher's site:

http://dx.doi.org/10.1111/ceo.12299 New Site Icon

Source:

Clin Experiment Ophthalmol. 43(4):358-366.

[PDF-400.39 KB]

Details:

Alternative Title:

Clin Experiment Ophthalmol

Personal Author:

de la Zerda, Adam ; Prabhulkar, Shradha ; Perez, Victor L ; Ruggeri, Marco ; Paranjape, Amit S ; ... More ▼

Description:

Background

Optical coherence tomography (OCT) is a powerful imaging modality to visualize tissue structures, with axial image pixel resolution as high as 1.6 μm in tissue. However, OCT is intrinsically limited to providing structural information as the OCT contrast is produced by optically scattering tissues.

Methods

Gold nanorods (GNRs) were injected into the anterior chamber (AC) and cornea of mice eyes which could create a significant OCT signal and hence could be used as a contrast agent for in vivo OCT imaging.

Results

A dose of 30 nM of GNRs (13 nm in diameter and 45 nm in length) were injected to the AC of mice eyes and produced an OCT contrast nearly 50-fold higher than control mice injected with saline. Furthermore, the lowest detectable concentration of GNRs in living mice AC was experimentally estimated to be as low as 120 pM.

Conclusions

The high sensitivity and low toxicity of GNRs brings great promise for OCT to uniquely become a high-resolution molecular imaging modality.

Subject:

[+]

Pubmed ID:

24533647

Pubmed Central ID:

PMC4963150

Document Type:

Journal Article

Funding:

DP5 0D012179/DP/NCCDPHP CDC HHS/United States ; DP5 OD012179/OD/NIH HHS/United States ; P50 CA114747/CA/NCI NIH HHS/United States ; P50 CA114747/CA/NCI NIH HHS/United States ; R21 EY020940/EY/NEI NIH HHS/United States ; ... More ▼

Collection(s):

CDC Public Access

Main Document Checksum:

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Supporting Files:

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