Biofunctionalization of Large Gold Nanorods Realizes Ultrahigh-Sensitivity Optical Imaging Agents

Details

• Alternative Title:
  Langmuir
• Personal Author:
  SoRelle, Elliott D. ; Liba, Orly ; Hussain, Zeshan ; Gambhir, Milan ; de la Zerda, Adam
• Description:
  Gold nanorods (GNRs, ∼ 50 × 15 nm) have been used ubiquitously in biomedicine for their optical properties, and many methods of GNR biofunctionalization have been described. Recently, the synthesis of larger-than-usual GNRs (LGNRs, ∼ 100 × 30 nm) has been demonstrated. However, LGNRs have not been biofunctionalized and therefore remain absent from biomedical literature to date. Here we report the successful biofunctionalization of LGNRs, which produces highly stable particles that exhibit a narrow spectral peak (FWHM ∼100 nm). We further demonstrated that functionalized LGNRs can be used as highly sensitive scattering contrast agents by detecting individual LGNRs in clear liquids. Owing to their increased optical cross sections, we found that LGNRs exhibited up to 32-fold greater backscattering than conventional GNRs. We leveraged these enhanced optical properties to detect LGNRs in the vasculature of live tumor-bearing mice. With LGNR contrast enhancement, we were able to visualize tumor blood vessels at depths that were otherwise undetectable. We expect that the particles reported herein will enable immediate sensitivity improvements in a wide array of biomedical imaging and sensing techniques that rely on conventional GNRs.
• Subjects:
  Animals Biotin Cell Line, Tumor Contrast Media Ear Female Glioblastoma Gold Light Mice Mice, Inbred C57BL Mice, Nude Nanotubes Neoplasm Transplantation Optical Imaging Particle Size Polyethylene Glycols Scattering, Radiation Streptavidin

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• Source:
  Langmuir. 31(45):12339-12347
• Pubmed ID:
  26477361
• Pubmed Central ID:
  PMC4963153
• Document Type:
  Journal Article
• Funding:
  T32 GM-08294/GM/NIGMS NIH HHSUnited States/ ; U54 CA151459/CA/NCI NIH HHSUnited States/ ; T32 GM008294/GM/NIGMS NIH HHSUnited States/ ; DP50D012179/DP/NCCDPHP CDC HHSUnited States/ ; 1S10RR026780001/RR/NCRR NIH HHSUnited States/ ; U54CA151459/CA/NCI NIH HHSUnited States/ ; DP5 OD012179/OD/NIH HHSUnited States/
• Volume:
  31
• Issue:
  45
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:23128dc3c535bc7e97a2dd6e2d3bf5f5526242ba51d07d938b1743c04ae129d5
• Download URL:
  https://stacks.cdc.gov/view/cdc/40551/cdc_40551_DS1.pdf
• File Type:
  [PDF - 1.43 MB ]