Details:

Alternative Title:
Org Lett
Publisher's site:
http://dx.doi.org/10.1021/ol401723h
Personal Author:
Winter, Jaclyn M. ; Chiou, Grace ; Bothwell, Ian R. ; Xu, Wei ; Garg, Neil K. ; ... More +
Winter, Jaclyn M. ; Chiou, Grace ; Bothwell, Ian R. ; Xu, Wei ; Garg, Neil K. ; Luo, Minkui ; Tang, Yi Less -
Description:
A strategy for introducing structural diversity into polyketides by exploiting the promiscuity of an in-line methyltransferase domain in a multidomain polyketide synthase is reported. In vitro investigations using the highly-reducing fungal polyketide synthase CazF revealed that its methyltransferase domain accepts the nonnatural cofactor propargylic Se-adenosyl-l-methionine and can transfer the propargyl moiety onto its growing polyketide chain. This propargylated polyketide product can then be further chain-extended and cyclized to form propargyl-α pyrone or be processed fully into the alkyne-containing 4'-propargyl-chaetoviridin A.
Subject:
[+]
Amino Acid Sequence Article Furans Methyltransferases Pargyline Polyketides Polyketide Synthases Protein Structure, Tertiary Pyrones
Source:
Org Lett. 15(14):3774-3777.
Pubmed ID:
23837609
Pubmed Central ID:
PMC3779521
Document Type:
Journal Article
Funding:
1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; 1DP2OD007335/OD/NIH HHS/United States ; 1R01GM096056/GM/NIGMS NIH HHS/United States ; DP1 GM106413/GM/NIGMS NIH HHS/United States ; DP2 OD007335/OD/NIH HHS/United States ; ... More +
1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; 1DP2OD007335/OD/NIH HHS/United States ; 1R01GM096056/GM/NIGMS NIH HHS/United States ; DP1 GM106413/GM/NIGMS NIH HHS/United States ; DP2 OD007335/OD/NIH HHS/United States ; R01 GM096056/GM/NIGMS NIH HHS/United States ; S10RR025631/RR/NCRR NIH HHS/United States ; T32 GM008496/GM/NIGMS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:95d0adf5be7d16056dc563d45523f04a3ad571b1704f70ea7e839f54fe44bea3
Download URL:
https://stacks.cdc.gov/view/cdc/29741/cdc_29741_DS1.pdf
File Type:

Supporting Files

• 	nihms504179f4.gif	gif
  	nihms504179f4.jpg	jpeg
  	nihms504179f5.gif	gif
  	nihms504179f5.jpg	jpeg
  	nihms504179u1.jpg	jpeg
  	NIHMS504179-supplement-1_si_001.pdf	pdf
  	nihms504179.nxml	txt
  	nihms504179f1.gif	gif
  	nihms504179f1.jpg	jpeg
  	nihms504179f2.gif	gif
  	nihms504179f2.jpg	jpeg
  	nihms504179f3.gif	gif
  	nihms504179f3.jpg	jpeg

More +