Identification and Characterization of the Chaetoviridin and Chaetomugilin Gene Cluster in Chaetomium globosum Reveals Dual Functions of an Iterative Highly-Reducing Polyketide Synthase

Winter, Jaclyn M.; Sato, Michio; Sugimoto, Satoru; Chiou, Grace; Garg, Neil K.; Tang, Yi; Watanabe, Kenji

doi:10.1021/ja3090498

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Identification and Characterization of the Chaetoviridin and Chaetomugilin Gene Cluster in Chaetomium globosum Reveals Dual Functions of an Iterative Highly-Reducing Polyketide Synthase

10 31 2012
By Winter, Jaclyn M. ; Sato, Michio ; Sugimoto, Satoru ; ...
http://dx.doi.org/10.1021/ja3090498
Source: J Am Chem Soc. 134(43):17900-17903

[PDF-975.83 KB]

English

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Details:

Alternative Title:

J Am Chem Soc
Personal Author:

Winter, Jaclyn M. ; Sato, Michio ; Sugimoto, Satoru ; Chiou, Grace ; Garg, Neil K. ; ... More +

Winter, Jaclyn M. ; Sato, Michio ; Sugimoto, Satoru ; Chiou, Grace ; Garg, Neil K. ; Tang, Yi ; Watanabe, Kenji Less -
Description:

We report the identification and characterization of the caz biosynthetic cluster from Chaetomium globosum and the characterization of a highly reducing polyketide synthase (PKS) that acts in both a sequential and convergent manner with a nonreducing PKS to form the chaetomugilin and chaetoviridin azaphilones. Genetic inactivation studies verified the involvement of individual caz genes in the biosynthesis of the azaphilones. Through in vitro reconstitution, we demonstrated the in vitro synthesis of chaetoviridin A from the pyranoquinone intermediate cazisochromene using the highly reducing PKS and an acyltransferase.
Subjects:

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Chaetomium Furans Molecular Structure Polyketide Synthases Pyrones
Keywords:

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Acyltransferase Azaphilone Biosynthesis Filamentous Fungi
Source:

J Am Chem Soc. 134(43):17900-17903
Pubmed ID:

23072467
Pubmed Central ID:

PMC3494086
Document Type:

Journal Article
Funding:

DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/ ; 1R01GM085128/GM/NIGMS NIH HHSUnited States/

DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/ ; 1R01GM085128/GM/NIGMS NIH HHSUnited States/ Less -
Collection(s):

CDC Public Access
Main Document Checksum:

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urn:sha256:1abc73f9e158cc77cf008e16f18f02cdbd889f72145287792947ace386452dae
Download URL:

https://stacks.cdc.gov/view/cdc/33468/cdc_33468_DS1.pdf
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