Details:

Alternative Title:
J Am Chem Soc
Publisher's site:
http://dx.doi.org/10.1021/ja3090498
Personal Author:
Winter, Jaclyn M. ; Sato, Michio ; Sugimoto, Satoru ; Chiou, Grace ; Garg, Neil K. ; ... More +
Winter, Jaclyn M. ; Sato, Michio ; Sugimoto, Satoru ; Chiou, Grace ; Garg, Neil K. ; Tang, Yi ; Watanabe, Kenji Less -
Description:
We report the identification and characterization of the caz biosynthetic cluster from Chaetomium globosum and the characterization of a highly reducing polyketide synthase (PKS) that acts in both a sequential and convergent manner with a nonreducing PKS to form the chaetomugilin and chaetoviridin azaphilones. Genetic inactivation studies verified the involvement of individual caz genes in the biosynthesis of the azaphilones. Through in vitro reconstitution, we demonstrated the in vitro synthesis of chaetoviridin A from the pyranoquinone intermediate cazisochromene using the highly reducing PKS and an acyltransferase.
Subjects:
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Acyltransferase Article Azaphilone Biosynthesis Chaetomium Filamentous Fungi Furans Molecular Structure Polyketide Synthases Pyrones
Source:
J Am Chem Soc. 134(43):17900-17903
Pubmed ID:
23072467
Pubmed Central ID:
PMC3494086
Document Type:
Journal Article
Funding:
DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/ ; 1R01GM085128/GM/NIGMS NIH HHSUnited States/
DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/ ; 1R01GM085128/GM/NIGMS NIH HHSUnited States/ Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:1abc73f9e158cc77cf008e16f18f02cdbd889f72145287792947ace386452dae
Download URL:
https://stacks.cdc.gov/view/cdc/33468/cdc_33468_DS1.pdf
File Type:

Supporting Files

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