Details:

Alternative Title:
J Am Chem Soc
Publisher's site:
http://dx.doi.org/10.1021/ja3090498
Personal Author:
Winter, Jaclyn M. ; Sato, Michio ; Sugimoto, Satoru ; Chiou, Grace ; Garg, Neil K. ; ... More +
Winter, Jaclyn M. ; Sato, Michio ; Sugimoto, Satoru ; Chiou, Grace ; Garg, Neil K. ; Tang, Yi ; Watanabe, Kenji ; Less -
Description:
We report the identification and characterization of the caz biosynthetic cluster from Chaetomium globosum and the characterization of a highly reducing polyketide synthase (PKS) that acts in both a sequential and convergent manner with a nonreducing PKS to form the chaetomugilin and chaetoviridin azaphilones. Genetic inactivation studies verified the involvement of individual caz genes in the biosynthesis of the azaphilones. Through in vitro reconstitution, we demonstrated the in vitro synthesis of chaetoviridin A from the pyranoquinone intermediate cazisochromene using the highly reducing PKS and an acyltransferase.
Subject:
[+]
Acyltransferase
Article
Azaphilone
Biosynthesis
Chaetomium
Filamentous Fungi
Furans
Molecular Structure
Polyketide Synthases
Pyrones

Source:
J Am Chem Soc. 134(43):17900-17903. ;
Pubmed ID:
23072467
Pubmed Central ID:
PMC3494086
Document Type:
Journal Article ;
Funding:
DP1 GM106413/GM/NIGMS NIH HHS/United States ; R01 GM085128/GM/NIGMS NIH HHS/United States ; 1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; 1R01GM085128/GM/NIGMS NIH HHS/United States ;
DP1 GM106413/GM/NIGMS NIH HHS/United States ; R01 GM085128/GM/NIGMS NIH HHS/United States ; 1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; 1R01GM085128/GM/NIGMS NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:1abc73f9e158cc77cf008e16f18f02cdbd889f72145287792947ace386452dae
File Type:

Supporting Files

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