Identification and Characterization of the Chaetoviridin and Chaetomugilin Gene Cluster in Chaetomium globosum Reveals Dual Functions of an Iterative Highly-Reducing Polyketide Synthase

Details

• Alternative Title:
  J Am Chem Soc
• Personal Author:
  Winter, Jaclyn M. ; Sato, Michio ; Sugimoto, Satoru ; Chiou, Grace ; Garg, Neil K. ; Tang, Yi ; Watanabe, Kenji
• Description:
  We report the identification and characterization of the caz biosynthetic cluster from Chaetomium globosum and the characterization of a highly reducing polyketide synthase (PKS) that acts in both a sequential and convergent manner with a nonreducing PKS to form the chaetomugilin and chaetoviridin azaphilones. Genetic inactivation studies verified the involvement of individual caz genes in the biosynthesis of the azaphilones. Through in vitro reconstitution, we demonstrated the in vitro synthesis of chaetoviridin A from the pyranoquinone intermediate cazisochromene using the highly reducing PKS and an acyltransferase.
• Subjects:
  • Chaetomium
  • Furans
  • Molecular Structure
  • Polyketide Synthases
  • Pyrones
• Keywords:
  • Azaphilone
  • filamentous fungi
  • acyltransferase
  • biosynthesis
• Source:
  J Am Chem Soc. 134(43):17900-17903
• Pubmed ID:
  23072467
• Pubmed Central ID:
  PMC3494086
• Document Type:
  Journal Article
• Funding:
  DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/ ; 1R01GM085128/GM/NIGMS NIH HHSUnited States/
• Volume:
  134
• Issue:
  43
• Download URL:
  https://stacks.cdc.gov/view/cdc/33468/cdc_33468_DS1.pdf
• File Type:
  [PDF - 975.83 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:1abc73f9e158cc77cf008e16f18f02cdbd889f72145287792947ace386452dae