Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq

Details

• Alternative Title:
  Nat Biotechnol
• Personal Author:
  Cadwell, Cathryn R ; Palasantza, Athanasia ; Jiang, Xiaolong ; Berens, Philipp ; Deng, Qiaolin ; Yilmaz, Marlene ; Reimer, Jacob ; Shen, Shan ; Bethge, Matthias ; Tolias, Kimberley F ; Sandberg, Rickard ; Tolias, Andreas S
• Description:
  Despite the importance of the mammalian neocortex for complex cognitive processes, we still lack a comprehensive description of its cellular components. To improve the classification of neuronal cell types and the functional characterization of single neurons, we present Patch-seq, a method that combines whole-cell electrophysiological patch-clamp recordings, single-cell RNA-sequencing and morphological characterization. Following electrophysiological characterization, cell contents are aspirated through the patch-clamp pipette and prepared for RNA-sequencing. Using this approach, we generate electrophysiological and molecular profiles of 58 neocortical cells and show that gene expression patterns can be used to infer the morphological and physiological properties such as axonal arborization and action potential amplitude of individual neurons. Our results shed light on the molecular underpinnings of neuronal diversity and suggest that Patch-seq can facilitate the classification of cell types in the nervous system.
• Subjects:
  Animals Article Cell Shape Female Gene Expression Profiling Male Mice Neocortex Neurons Patch-Clamp Techniques Sequence Analysis, RNA Transcriptome

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• Source:
  Nat Biotechnol. 34(2):199-203.
• Pubmed ID:
  26689543
• Pubmed Central ID:
  PMC4840019
• Document Type:
  Journal Article
• Funding:
  DP1EY023176/DP/NCCDPHP CDC HHS/United States ; DP1OD008301/OD/NIH HHS/United States ; F30 MH095440/MH/NIMH NIH HHS/United States ; F30MH095440/MH/NIMH NIH HHS/United States ; P30EY002520/EY/NEI NIH HHS/United States ; R01 MH103108/MH/NIMH NIH HHS/United States ; R01MH103108/MH/NIMH NIH HHS/United States ; R01NS062829/NS/NINDS NIH HHS/United States ; T32 GM007330/GM/NIGMS NIH HHS/United States ; T32EB006350/EB/NIBIB NIH HHS/United States ; T32EY07001/EY/NEI NIH HHS/United States ; T32GM007330/GM/NIGMS NIH HHS/United States
• Volume:
  34
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:3d304f9f62571ce58c4fa640160b206cd3044ff15eae6e27041a9aaa12c1b5e2
• Download URL:
  https://stacks.cdc.gov/view/cdc/39773/cdc_39773_DS1.pdf
• File Type:
  [PDF - 558.26 KB ]