Details:

Alternative Title:
Nat Methods
Personal Author:
Lovatt, Ditte ; Ruble, Brittani K. ; Lee, Jaehee ; Dueck, Hannah ; Kim, Tae Kyung ; ... More +
Lovatt, Ditte ; Ruble, Brittani K. ; Lee, Jaehee ; Dueck, Hannah ; Kim, Tae Kyung ; Fisher, Stephen ; Francis, Chantal ; Spaethling, Jennifer M. ; Wolf, John A. ; Grady, M. Sean ; Ulyanova, Alexandra V. ; Yeldell, Sean B. ; Griepenburg, Julianne C. ; Buckley, Peter T. ; Kim, Junhyong ; Sul, Jai-Yoon ; Dmochowski, Ivan J. ; Eberwine, James Less -
Description:
Transcriptome profiling of single cells resident in their natural microenvironment depends upon RNA capture methods that are both noninvasive and spatially precise. We engineered a transcriptome in vivo analysis (TIVA) tag, which upon photoactivation enables mRNA capture from single cells in live tissue. Using the TIVA tag in combination with RNA sequencing (RNA-seq), we analyzed transcriptome variance among single neurons in culture and in mouse and human tissue in vivo. Our data showed that the tissue microenvironment shapes the transcriptomic landscape of individual cells. The TIVA methodology is, to our knowledge, the first noninvasive approach for capturing mRNA from live single cells in their natural microenvironment.
Subjects:
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Animals Article Brain Computational Biology Gene Expression Profiling Gene Library High-Throughput Nucleotide Sequencing Hippocampus Humans Mice Mice, Inbred C57BL Neurons RNA, Messenger Sequence Analysis, RNA
Source:
Nat Methods. 11(2):190-196.
Pubmed ID:
24412976
Pubmed Central ID:
PMC3964595
Document Type:
Journal Article
Funding:
DP004117/DP/NCCDPHP CDC HHS/United States ; DP1 MH099902/MH/NIMH NIH HHS/United States ; DP1 OD004117/OD/NIH HHS/United States ; R01 GM083030/GM/NIGMS NIH HHS/United States ; R01 GM083030/GM/NIGMS NIH HHS/United States ; ... More +
DP004117/DP/NCCDPHP CDC HHS/United States ; DP1 MH099902/MH/NIMH NIH HHS/United States ; DP1 OD004117/OD/NIH HHS/United States ; R01 GM083030/GM/NIGMS NIH HHS/United States ; R01 GM083030/GM/NIGMS NIH HHS/United States ; S10 RR021113/RR/NCRR NIH HHS/United States ; U01 MH098953/MH/NIMH NIH HHS/United States ; U01MH098953/MH/NIMH NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:5f6e9dae0c29c4490c351523f935c1aa075496e786cb775515efe6cfce1aec2f
Download URL:
https://stacks.cdc.gov/view/cdc/24457/cdc_24457_DS1.pdf
File Type:

Supporting Files

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