Plasmodium falciparum K76T pfcrt Gene Mutations and Parasite Population Structure, Haiti, 2006–2009

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Charles, Macarthur ; Das, Sanchita ; Daniels, Rachel ; Kirkman, Laura ; Delva, Glavdia G. ; Destine, Rodney ; Escalante, Ananias ; Villegas, Leopoldo ; Daniels, Noah M. ; Shigyo, Kristi ; Volkman, Sarah K. ; Pape, Jean W. ; Golightly, Linnie M.
• Description:
  Hispaniola is the only Caribbean island to which Plasmodium falciparum malaria remains endemic. Resistance to the antimalarial drug chloroquine has rarely been reported in Haiti, which is located on Hispaniola, but the K76T pfcrt (P. falciparum chloroquine resistance transporter) gene mutation that confers chloroquine resistance has been detected intermittently. We analyzed 901 patient samples collected during 2006-2009 and found 2 samples showed possible mixed parasite infections of genetically chloroquine-resistant and -sensitive parasites. Direct sequencing of the pfcrt resistance locus and single-nucleotide polymorphism barcoding did not definitively identify a resistant population, suggesting that sustained propagation of chloroquine-resistant parasites was not occurring in Haiti during the study period. Comparison of parasites from Haiti with those from Colombia, Panama, and Venezuela reveals a geographically distinct population with highly related parasites. Our findings indicate low genetic diversity in the parasite population and low levels of chloroquine resistance in Haiti, raising the possibility that reported cases may be of exogenous origin.
• Subjects:
  Dna Barcoding, Taxonomic Geography History, 21St Century Humans Malaria, Falciparum/Epidemiology Malaria, Falciparum/History Malaria, Falciparum/Parasitology Membrane Transport Proteins Mutation Phylogeography Plasmodium Falciparum Protozoan Proteins Sequence Analysis, Dna

  More +
• Source:
  Emerg Infect Dis. 22(5):786-793.
• Pubmed ID:
  27089479
• Pubmed Central ID:
  PMC4861504
• Document Type:
  Journal Article
• Genre:
  Historical Article
• Place as Subject:
  Haiti
• Volume:
  22
• Issue:
  5
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:52bb76881200730f2e756c98e525d06563c56c189e46136fa35fc6cbc1205f72
• Download URL:
  https://stacks.cdc.gov/view/cdc/39352/cdc_39352_DS1.pdf
• File Type:
  [PDF - 713.90 KB ]