Details:

Alternative Title:
Clin Biochem
Publisher's site:
http://dx.doi.org/10.1016/j.clinbiochem.2015.09.011
Personal Author:
Haynes, Christopher A. ; De Jesús, Víctor R. ;
Haynes, Christopher A. ; De Jesús, Víctor R. ; Less -
Description:
Objectives The goal of this study was to include the quantitation of hexacosanoyl lysophosphatidylcholine, a biomarker for X-linked adrenoleukodystrophy and other peroxisomal disorders, in the routine extraction and analysis procedure used to quantitate amino acids, acylcarnitines, and succinylacetone during newborn screening. Criteria for the method included use of a single punch from a dried blood spot, one simple extraction of the punch, no high-performance liquid chromatography, and utilizing tandem mass spectrometry to quantitate the analytes. Design and methods Dried blood spot punches were extracted with a methanolic solution of stable-isotope labeled internal standards, formic acid, and hydrazine, followed by flow injection analysis–electrospray ionization–tandem mass spectrometry. Results Quantitation of amino acids, acylcarnitines, and hexacosanoyl lysophosphatidylcholine using this combined method was similar to results obtained using two separate methods. Conclusions A single dried blood spot punch extracted by a rapid (45 min), simple procedure can be analyzed with high throughput (2 min per sample) to quantitate amino acids, acylcarnitines, succinylacetone, and hexacosanoyl lysophosphatidylcholine.
Subject:
[+]
Article
Dried Blood Spot
Flow-injection Analysis
Newborn Screening
Tandem Mass Spectrometry
X-linked Adrenoleukodystrophy

Source:
Clin Biochem. 49(1):161-165. ;
Pubmed ID:
26432925
Pubmed Central ID:
PMC4836612
Document Type:
Journal Article ;
Funding:
CC999999/Intramural CDC HHS/United States ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:c100fd1a0f011335e37c43efca71e70960e9d55d70a6a7e406a3e5021f3e5d80
File Type:

Supporting Files

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  	NIHMS775325-supplement-Supplementary_Table_I.docx	docx

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