The stability of hexacosanoyl lysophosphatidylcholine in dried-blood spot quality control materials for X-linked adrenoleukodystrophy newborn screening

Details

• Alternative Title:
  Clin Biochem
• Personal Author:
  Haynes, Christopher A. ; De Jesús, Víctor R.
• Description:
  Objectives
  
  Newborn screening for X-linked adrenoleukodystrophy utilizes tandem mass spectrometry to analyze dried-blood spot specimens. Quality control materials (dried-blood spots enriched with hexacosanoyl lysophosphatidylcholine) were prepared and stored at different temperatures for up to 518 days to evaluate the stability of this biomarker for X-linked adrenoleukodystrophy.
  
  Design and methods
  
  Dried-blood spot storage included desiccant (45, 171, and 518 days) or omitted desiccant (53 days at >90% relative humidity). Specimens were stored for 171 and 518 days at −20 °C, 4 °C, ambient temperature, and 37 °C. Each weekday for 45 days, a bag of specimens stored at 4 °C was warmed to ambient temperature and one specimen was removed for storage at −80 °C. Specimens were analyzed by high-performance liquid-chromatography electrospray ionization tandem mass spectrometry and data was plotted as concentration (micromoles per liter) vs. time. Linear regression provided slope and y-intercept values for each storage condition.
  
  Results
  
  Small slope values (0.01 or less) and y-intercept values close to the enrichment indicated less than 11% loss of hexacosanoyl lysophosphatidylcholine under all storage conditions tested.
  
  Conclusions
  
  Quality control materials for X-linked adrenoleukodystrophy are stable for at least 1 year when stored with desiccant.
  
  
• Subjects:
  Adrenoleukodystrophy Article Dried-blood Spot Dried Blood Spot Testing Humans Infant, Newborn Lysophosphatidylcholine Lysophosphatidylcholines Neonatal Screening Newborn Screening Quality Control Tandem Mass Spectrometry X-linked Adrenoleukodystrophy

  More +
• Source:
  Clin Biochem. 48(1-2):8-10.
• Pubmed ID:
  25307302
• Pubmed Central ID:
  PMC4835029
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  48
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:13058aeac042a6ea24e300f5813aa96f54ddd9e7b6741b478b8ff4bcfcb02095
• Download URL:
  https://stacks.cdc.gov/view/cdc/38978/cdc_38978_DS1.pdf
• File Type:
  [PDF - 83.55 KB ]