CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.
As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
i
Multiplex Single Cell Profiling of Chromatin Accessibility by Combinatorial Cellular Indexing
-
5 22 2015
-
-
Source: Science. 348(6237):910-914
[PDF-1.12 MB]
Details:
-
Alternative Title:Science
-
Personal Author:
-
Description:Technical advances have enabled the collection of genome and transcriptome data sets with single-cell resolution. However, single-cell characterization of the epigenome has remained challenging. Furthermore, because cells must be physically separated before biochemical processing, conventional single-cell preparatory methods scale linearly. We applied combinatorial cellular indexing to measure chromatin accessibility in thousands of single cells per assay, circumventing the need for compartmentalization of individual cells. We report chromatin accessibility profiles from more than 15,000 single cells and use these data to cluster cells on the basis of chromatin accessibility landscapes. We identify modules of coordinately regulated chromatin accessibility at the level of single cells both between and within cell types, with a scalable method that may accelerate progress toward a human cell atlas.
-
Subjects:
-
Source:
-
Pubmed ID:25953818
-
Pubmed Central ID:PMC4836442
-
Document Type:
-
Funding:
-
Volume:348
-
Issue:6237
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
