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Fixed single-cell transcriptomic characterization of human radial glial diversity
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Published Date:
Nov 16 2015
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Source:Nat Methods. 13(1):87-93.
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[PDF-2.92 MB]
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Alternative Title:Nat Methods
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Description:The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of the midgestation cortex and classified them as ventricular zone-enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that express HOPX. Our study identified vRG and oRG markers and molecular profiles, an essential step for understanding human neocortical progenitor development. FRISCR allows targeted single-cell profiling of any tissues that lack live-cell markers.
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Subject:
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Pubmed ID:26524239
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Pubmed Central ID:PMC4869711
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