Fixed single-cell transcriptomic characterization of human radial glial diversity

Details

• Alternative Title:
  Nat Methods
• Personal Author:
  Thomsen, Elliot R. ; Mich, John K. ; Yao, Zizhen ; Hodge, Rebecca D. ; Doyle, Adele M. ; Jang, Sumin ; Shehata, Soraya I. ; Nelson, Angelique M. ; Shapovalova, Nadiya V. ; Levi, Boaz P. ; Ramanathan, Sharad
• Description:
  The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of the midgestation cortex and classified them as ventricular zone-enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that express HOPX. Our study identified vRG and oRG markers and molecular profiles, an essential step for understanding human neocortical progenitor development. FRISCR allows targeted single-cell profiling of any tissues that lack live-cell markers.
• Subjects:
  Article Brain Humans Neuroglia Single-Cell Analysis Transcriptome
• Source:
  Nat Methods. 13(1):87-93.
• Pubmed ID:
  26524239
• Pubmed Central ID:
  PMC4869711
• Document Type:
  Journal Article
• Funding:
  5DP1MH099906-03/DP/NCCDPHP CDC HHS/United States ; 5R24HD000836/HD/NICHD NIH HHS/United States ; DP1 MH099906/MH/NIMH NIH HHS/United States ; R24 HD000836/HD/NICHD NIH HHS/United States
• Volume:
  13
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:21e620ead97fae48751d24f2e6861465780ef3ba5d6d5a906648caa92afb6bb1
• Download URL:
  https://stacks.cdc.gov/view/cdc/39492/cdc_39492_DS1.pdf
• File Type:
  [PDF - 2.92 MB ]