Fixed single-cell transcriptomic characterization of human radial glial diversity

Thomsen, Elliot R.; Mich, John K.; Yao, Zizhen; Hodge, Rebecca D.; Doyle, Adele M.; Jang, Sumin; Shehata, Soraya I.; Nelson, Angelique M.; Shapovalova, Nadiya V.; Levi, Boaz P.; Ramanathan, Sharad

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Fixed single-cell transcriptomic characterization of human radial glial diversity

Published Date:

Nov 16 2015

Source:

Nat Methods. 13(1):87-93.

[PDF - 2.92 MB]

Details:

Personal Authors:

Thomsen, Elliot R. ; Mich, John K. ; Yao, Zizhen ; Hodge, Rebecca D. ; Doyle, Adele M. ; ... More ▼

Keywords:

[+]

Pubmed ID:

26524239

Pubmed Central ID:

PMC4869711

Description:

The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of the midgestation cortex and classified them as ventricular zone-enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that express HOPX. Our study identified vRG and oRG markers and molecular profiles, an essential step for understanding human neocortical progenitor development. FRISCR allows targeted single-cell profiling of any tissues that lack live-cell markers.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

5DP1MH099906-03/DP/NCCDPHP CDC HHS/United States
5R24HD000836/HD/NICHD NIH HHS/United States
DP1 MH099906/MH/NIMH NIH HHS/United States
R24 HD000836/HD/NICHD NIH HHS/United States

Supporting Files:

NIHMS727861-supplement-1.doc	application/msword
NIHMS727861-supplement-2.xlsx	application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
NIHMS727861-supplement-suppdataset2.xlsx	application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
NIHMS727861-supplement-suppdataset_1.xlsx	application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
nihms727861.nxml	application/octet-stream
nihms727861f1.gif	image/gif
nihms727861f1.jpg	image/jpeg
nihms727861f2.gif	image/gif
nihms727861f2.jpg	image/jpeg
nihms727861f3.gif	image/gif
nihms727861f3.jpg	image/jpeg
nihms727861f4.gif	image/gif
nihms727861f4.jpg	image/jpeg
nihms727861f5.gif	image/gif
nihms727861f5.jpg	image/jpeg
nihms727861f6.gif	image/gif
nihms727861f6.jpg	image/jpeg

No Related Documents.

You May Also Like:

Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq

Personal Author:

Cadwell, Cathryn R; Palasantza, Athanasia; Jiang, Xiaolong; Berens, Philipp; Deng, Qiaolin; Yilmaz, Marlene; Reimer, Jacob; Shen, Shan; Bethge, Matthias; Tolias, Kimberley F; Sandberg, Rickard; Tolias, Andreas S;

Published:

Dec 21 2015

Source:

Nat Biotechnol. 34(2):199-203.

Description:

Despite the importance of the mammalian neocortex for complex cognitive processes, we still lack a comprehensive description of its cellular components. To improve the classification of neuronal cell types and the functional characterization of singl...

File Type:

[PDF - 558.26 KB]
Transcriptomic Characterization of the Novel Avian-Origin Influenza A (H7N9) Virus: Specific Host Response and Responses Intermediate between Avian (H5N1 and H7N7) and Human (H3N2) Viruses and Implications for Treatment Options

Personal Author:

Josset, Laurence; Zeng, Hui; Kelly, Sara M.; Tumpey, Terrence M.; Katze, Michael G.;

Published:

Feb 04 2014

Source:

mBio. 2014; 5(1).

Description:

A novel avian-origin H7N9 influenza A virus (IAV) emerged in China in 2013, causing mild to lethal human respiratory infections. H7N9 originated with multiple reassortment events between avian viruses and carries genetic markers of human adaptation. ...

File Type:

[PDF - 2.64 MB]
Antigenic Diversity of Human Sapoviruses

Personal Author:

Hansman, Grant S.; Oka, Tomoichiro; Sakon, Naomi; Takeda, Naokazu;

Published:

Oct 2007

Source:

Emerg Infect Dis. 13(10):1519-1525.

Description:

Sapovirus (SaV) is a causative agent of gastroenteritis. On the basis of capsid protein (VP1) nucleotide sequences, SaV can be divided into 5 genogroups (GI-GV), of which the GI, GII, GIV, and GV strains infect humans. SaV is uncultivable, but expres...

File Type:

[PDF - 323.96 KB]
Structural characterization of human Uch37.

Personal Author:

Burgie, E. Sethe; Bingman, Craig A.; Soni, Ameet B.; Phillips, George N.;

Published:

Sep 26 2011

Source:

Proteins. 80(2):649-654.

Description:

Uch37 is a de-ubiquitylating enzyme that is functionally linked with the 26S proteasome via Rpn13, and is essential for metazoan development. Here, we report the X-ray crystal structure of full-length human Uch37 at 2.95 Å resolution. Uch37's c...

File Type:

[PDF - 771.85 KB]
Multiplex Single Cell Profiling of Chromatin Accessibility by Combinatorial Cellular Indexing

Personal Author:

Cusanovich, Darren A.; Daza, Riza; Adey, Andrew; Pliner, Hannah; Christiansen, Lena; Gunderson, Kevin L.; Steemers, Frank J.; Trapnell, Cole; Shendure, Jay;

Published:

May 07 2015

Source:

Science. 348(6237):910-914.

Description:

Technical advances have enabled the collection of genome and transcriptome data sets with single-cell resolution. However, single-cell characterization of the epigenome has remained challenging. Furthermore, because cells must be physically separated...

File Type:

[PDF - 1.12 MB]