Fixed single-cell transcriptomic characterization of human radial glial diversity

Thomsen, Elliot R.; Mich, John K.; Yao, Zizhen; Hodge, Rebecca D.; Doyle, Adele M.; Jang, Sumin; Shehata, Soraya I.; Nelson, Angelique M.; Shapovalova, Nadiya V.; Levi, Boaz P.; Ramanathan, Sharad

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Fixed single-cell transcriptomic characterization of human radial glial diversity

Published Date:

Nov 16 2015

Source:

Nat Methods. 13(1):87-93.

[PDF-2.92 MB]

Details:

Personal Authors:

Thomsen, Elliot R. ; Mich, John K. ; Yao, Zizhen ; Hodge, Rebecca D. ; Doyle, Adele M. ; ... More ▼

Keywords:

[+]

Pubmed ID:

26524239

Pubmed Central ID:

PMC4869711

Description:

The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of the midgestation cortex and classified them as ventricular zone-enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that express HOPX. Our study identified vRG and oRG markers and molecular profiles, an essential step for understanding human neocortical progenitor development. FRISCR allows targeted single-cell profiling of any tissues that lack live-cell markers.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

5DP1MH099906-03/DP/NCCDPHP CDC HHS/United States
5R24HD000836/HD/NICHD NIH HHS/United States
DP1 MH099906/MH/NIMH NIH HHS/United States
R24 HD000836/HD/NICHD NIH HHS/United States

Supporting Files:

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