Validation of biomarkers of CVD risk from dried blood spots in community-based research: Methodologies and study-specific serum equivalencies

Details

• Alternative Title:
  Biodemography Soc Biol
• Personal Author:
  Samuelsson, Laura B. ; Hall, Martica H. ; McLean, Shakir ; Porter, James H. ; Berkman, Lisa ; Marino, Miguel ; Sembajwe, Grace ; McDade, Thomas W. ; Buxton, Orfeu M.
• Description:
  Objective
  
  Dried blood spot (DBS) methodology offers significant advantages over venipuncture in vulnerable populations or large-scale studies, including reduced participant burden and higher response rates. Uncertainty about validity of cardiovascular risk biomarkers remains a barrier to wide-scale use. We determined the validity of DBS-derived biomarkers of CVD risk versus gold-standard assessments, and study-specific, serum-equivalency values for clinical relevance of DBS-derived values.
  
  Methods
  
  Concurrent venipuncture serum and DBS samples (n=150 adults) were assayed in CLIA-certified and DBS laboratories, respectively. Time controls of DBS standard samples were assayed single-blind along with test samples. Linear regression analyses evaluated DBS-to-serum equivalency values; agreement and bias were assessed via Bland-Altman plots.
  
  Results
  
  Linear regressions of venipuncture values on DBS-to-serum equivalencies provided R2 values for TC, HDL-C, CRP of 0.484, 0.118, 0.666, respectively. Bland-Altman plots revealed minimal systematic bias between DBS-to-serum and venipuncture values; precision worsened at higher mean values of CRP. Time controls reveal little degradation or change in analyte values for HDL-C and CRP over 30 weeks.
  
  Conclusions
  
  DBS-assessed biomarkers represent a valid alternative to venipuncture assessments. Large studies using DBS should include study-specific serum-equivalency determinations to optimize individual-level sensitivity, viability of detecting intervention effects, and generalizability in community-level, primary prevention interventions.
  
  
• Subjects:
  Adult Aged Biomarkers C-Reactive Protein Cardiovascular Diseases Cholesterol Cholesterol, HDL Dried Blood Spot Testing Female Humans Male Middle Aged Quality Control Regression Analysis Reproducibility Of Results Young Adult

  More +
• Keywords:
  Biomarkers Cardiovascular Disease Risk Cholesterol CRP Dried Blood Spots
• Source:
  Biodemography Soc Biol. 61(3):285-297
• Pubmed ID:
  26652683
• Pubmed Central ID:
  PMC4812568
• Document Type:
  Journal Article
• Funding:
  UL1 TR000005/TR/NCATS NIH HHSUnited States/ ; UL1 RR024153/RR/NCRR NIH HHSUnited States/ ; U19OH008861/ACL/ACL HHSUnited States/ ; U01-AG027669/AG/NIA NIH HHSUnited States/ ; T32-HL007901/HL/NHLBI NIH HHSUnited States/ ; R01-HL107240/HL/NHLBI NIH HHSUnited States/ ; UL1 RR025758/RR/NCRR NIH HHSUnited States/ ; U01 AG027669/AG/NIA NIH HHSUnited States/ ; R01 HL107240/HL/NHLBI NIH HHSUnited States/ ; UL1-TR000005/TR/NCATS NIH HHSUnited States/ ; UL1-RR024153/RR/NCRR NIH HHSUnited States/ ; R01-HL104607/HL/NHLBI NIH HHSUnited States/ ; T32 HL007901/HL/NHLBI NIH HHSUnited States/ ; R01 HL104607/HL/NHLBI NIH HHSUnited States/ ; T32 HL007560/HL/NHLBI NIH HHSUnited States/ ; U19 OH008861/OH/NIOSH CDC HHSUnited States/
• Volume:
  61
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:b49de05e61b652047f18d415d900734c775ce1196fd6c5669c58cda3c43e4fe5
• Download URL:
  https://stacks.cdc.gov/view/cdc/38750/cdc_38750_DS1.pdf
• File Type:
  [PDF - 1.69 MB ]