Details:

Alternative Title:
Biodemography Soc Biol
Publisher's site:
http://dx.doi.org/10.1080/19485565.2015.1068105
Personal Author:
Samuelsson, Laura B. ; Hall, Martica H. ; McLean, Shakir ; Porter, James H. ; Berkman, Lisa ; ... More +
Samuelsson, Laura B. ; Hall, Martica H. ; McLean, Shakir ; Porter, James H. ; Berkman, Lisa ; Marino, Miguel ; Sembajwe, Grace ; McDade, Thomas W. ; Buxton, Orfeu M. ; Less -
Description:
Objective Dried blood spot (DBS) methodology offers significant advantages over venipuncture in vulnerable populations or large-scale studies, including reduced participant burden and higher response rates. Uncertainty about validity of cardiovascular risk biomarkers remains a barrier to wide-scale use. We determined the validity of DBS-derived biomarkers of CVD risk versus gold-standard assessments, and study-specific, serum-equivalency values for clinical relevance of DBS-derived values. Methods Concurrent venipuncture serum and DBS samples (n=150 adults) were assayed in CLIA-certified and DBS laboratories, respectively. Time controls of DBS standard samples were assayed single-blind along with test samples. Linear regression analyses evaluated DBS-to-serum equivalency values; agreement and bias were assessed via Bland-Altman plots. Results Linear regressions of venipuncture values on DBS-to-serum equivalencies provided R2 values for TC, HDL-C, CRP of 0.484, 0.118, 0.666, respectively. Bland-Altman plots revealed minimal systematic bias between DBS-to-serum and venipuncture values; precision worsened at higher mean values of CRP. Time controls reveal little degradation or change in analyte values for HDL-C and CRP over 30 weeks. Conclusions DBS-assessed biomarkers represent a valid alternative to venipuncture assessments. Large studies using DBS should include study-specific serum-equivalency determinations to optimize individual-level sensitivity, viability of detecting intervention effects, and generalizability in community-level, primary prevention interventions.
Subject:
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Article
Biomarkers
Cardiovascular Disease Risk
Cholesterol
CRP
Dried Blood Spots

Source:
Biodemography Soc Biol. 61(3):285-297.
Pubmed ID:
26652683
Pubmed Central ID:
PMC4812568
Document Type:
Journal Article ;
Funding:
R01 HL104607/HL/NHLBI NIH HHS/United States ; R01 HL107240/HL/NHLBI NIH HHS/United States ; R01-HL104607/HL/NHLBI NIH HHS/United States ; R01-HL107240/HL/NHLBI NIH HHS/United States ; T32 HL007901/HL/NHLBI NIH HHS/United States ; ... More +
R01 HL104607/HL/NHLBI NIH HHS/United States ; R01 HL107240/HL/NHLBI NIH HHS/United States ; R01-HL104607/HL/NHLBI NIH HHS/United States ; R01-HL107240/HL/NHLBI NIH HHS/United States ; T32 HL007901/HL/NHLBI NIH HHS/United States ; T32-HL007901/HL/NHLBI NIH HHS/United States ; U01 AG027669/AG/NIA NIH HHS/United States ; U01-AG027669/AG/NIA NIH HHS/United States ; U19 OH008861/OH/NIOSH CDC HHS/United States ; U19 OH008861/OH/NIOSH CDC HHS/United States ; UL1 RR024153/RR/NCRR NIH HHS/United States ; UL1 RR025758/RR/NCRR NIH HHS/United States ; UL1 TR000005/TR/NCATS NIH HHS/United States ; UL1-RR024153/RR/NCRR NIH HHS/United States ; UL1-TR000005/TR/NCATS NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:b49de05e61b652047f18d415d900734c775ce1196fd6c5669c58cda3c43e4fe5
File Type:

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