Validation of biomarkers of CVD risk from dried blood spots in community-based research: Methodologies and study-specific serum equivalencies

Samuelsson, Laura B.; Hall, Martica H.; McLean, Shakir; Porter, James H.; Berkman, Lisa; Marino, Miguel; Sembajwe, Grace; McDade, Thomas W.; Buxton, Orfeu M.

doi:10.1080/19485565.2015.1068105

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Validation of biomarkers of CVD risk from dried blood spots in community-based research: Methodologies and study-specific serum equivalencies

Published Date:

2015

Publisher's site:

http://dx.doi.org/10.1080/19485565.2015.1068105 New Site Icon

Source:

Biodemography Soc Biol. 61(3):285-297.

[PDF-1.69 MB]

Details:

Personal Authors:

Samuelsson, Laura B. ; Hall, Martica H. ; McLean, Shakir ; Porter, James H. ; Berkman, Lisa ; ... More ▼

Keywords:

[+]

Pubmed ID:

26652683

Pubmed Central ID:

PMC4812568

Description:

Objective

Dried blood spot (DBS) methodology offers significant advantages over venipuncture in vulnerable populations or large-scale studies, including reduced participant burden and higher response rates. Uncertainty about validity of cardiovascular risk biomarkers remains a barrier to wide-scale use. We determined the validity of DBS-derived biomarkers of CVD risk versus gold-standard assessments, and study-specific, serum-equivalency values for clinical relevance of DBS-derived values.

Methods

Concurrent venipuncture serum and DBS samples (n=150 adults) were assayed in CLIA-certified and DBS laboratories, respectively. Time controls of DBS standard samples were assayed single-blind along with test samples. Linear regression analyses evaluated DBS-to-serum equivalency values; agreement and bias were assessed via Bland-Altman plots.

Results

Linear regressions of venipuncture values on DBS-to-serum equivalencies provided R2 values for TC, HDL-C, CRP of 0.484, 0.118, 0.666, respectively. Bland-Altman plots revealed minimal systematic bias between DBS-to-serum and venipuncture values; precision worsened at higher mean values of CRP. Time controls reveal little degradation or change in analyte values for HDL-C and CRP over 30 weeks.

Conclusions

DBS-assessed biomarkers represent a valid alternative to venipuncture assessments. Large studies using DBS should include study-specific serum-equivalency determinations to optimize individual-level sensitivity, viability of detecting intervention effects, and generalizability in community-level, primary prevention interventions.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

Supporting Files:

	nihms743426f3.jpg	image/jpeg
	nihms743426f4.gif	image/gif
	nihms743426f4.jpg	image/jpeg
	nihms743426f5.gif	image/gif
	nihms743426f5.jpg	image/jpeg
	nihms743426.nxml	application/octet-stream
	nihms743426f1.gif	image/gif
	nihms743426f1.jpg	image/jpeg
	nihms743426f2a.gif	image/gif
	nihms743426f2a.jpg	image/jpeg
	nihms743426f2b.gif	image/gif
	nihms743426f2b.jpg	image/jpeg
	nihms743426f3.gif	image/gif

No Related Documents.

You May Also Like:

Work-family conflict, cardiometabolic risk and sleep duration in nursing employees

Personal Author:

Berkman, Lisa F.; Liu, Sze Yan; Hammer, Leslie; Moen, Phyllis; Klein, Laura Cousino; Kelly, Erin; Fay, Martha; Davis, Kelly; Durham, Mary; Karuntzos, Georgia; Buxton, Orfeu M.;

Published:

May 11 2015

Source:

J Occup Health Psychol. 20(4):420-433

Description:

We investigated associations of work-family conflict and work and family conditions with objectively measured cardiometabolic risk and sleep. Multilevel analyses assessed cross-sectional associations between employee and job characteristics and healt...

File Type:

[PDF - 586.80 KB]
Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Personal Author:

Ostler, Michael W.; Porter, James H.; Buxton, Orfeu M.;

Published:

Jan 28 2014

Source:

J Vis Exp. 2014; (83).

Description:

Biomarkers are directly-measured biological indicators of disease, health, exposures, or other biological information. In population and social sciences, biomarkers need to be easy to obtain, transport, and analyze. Dried Blood Spots meet this need, ...

File Type:

[PDF - 331.29 KB]
Integrated Serologic Surveillance of Population Immunity and Disease Transmission

Personal Author:

Arnold, Benjamin F.; Scobie, Heather M.; Priest, Jeffrey W.; Lammie, Patrick J.;

Published:

Jul 2018

Source:

Emerg Infect Dis. 24(7):1188-1194.

Description:

Antibodies are unique among biomarkers in their ability to identify persons with protective immunity to vaccine-preventable diseases and to measure past exposure to diverse pathogens. Most infectious disease surveillance maintains a single-disease fo...

File Type:

[PDF - 2.44 MB]
Evaluation of Dried Blood Spots with a Multiplex Assay for Measuring Recent HIV-1 Infection

Personal Author:

Curtis, Kelly A.; Ambrose, Krystin M.; Kennedy, M. Susan; Owen, S. Michele;

Published:

Sep 18 2014

Source:

PLoS One. 2014; 9(9).

Description:

Laboratory-based HIV tests for recent infection (TRIs), which primarily measure a specific serological biomarker(s) that distinguishes recent from long-term HIV infection, have facilitated the estimation of population-based incidence. Dried blood spo...

File Type:

[PDF - 276.67 KB]
Enzymatic Screening and Diagnosis of Lysosomal Storage Diseases

Personal Author:

Yu, Chunli; Sun, Qin; Zhou, Hui;

Published:

2013

Source:

N Am J Med Sci (Boston). 6(4):186-193.

Description:

Lysosomal storage diseases (LSDs) are a group of more than 50 genetic disorders. Clinical symptoms are caused by the deficiency of specific enzyme (enzymes) function and resultant substrate accumulation in the lysosomes, which leads to impaired cellu...

File Type:

[PDF - 370.62 KB]