LDL cholesterol performance of beta quantification reference measurement procedure☆

Details

• Alternative Title:
  Clin Chim Acta
• Personal Author:
  Nakamura, Masakazu ; Kayamori, Yuzo ; Iso, Hiroyasu ; Kitamura, Akihiko ; Kiyama, Masahiko ; Koyama, Isao ; Nishimura, Kunihiro ; Nakai, Michikazu ; Noda, Hiroyuki ; Dasti, Mahnaz ; Vesper, Hubert W. ; Miyamoto, Yoshihiro
• Description:
  Background
  
  Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements forover 20 years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP.
  
  Methods
  
  Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15 years. Data were compared statistically using method comparison and bias estimation analysis.
  
  Results
  
  Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y = 0.988x + 1.794 (R2 = 0.997), y = 0.980x + 1.118 (R2 = 0.994), and y = 0.987x + 1.200 (R2 = 0.997), respectively. The Osaka laboratory met performance goals for 90% to 95% of the CDC reference values.
  
  Conclusions
  
  The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15 years. Accurate measurement of BFC is critical for the determination of LDL-C.
  
  
• Subjects:
  Cholesterol, HDL Cholesterol, LDL Humans Reference Standards Regression Analysis Reproducibility Of Results Ultracentrifugation
• Keywords:
  Beta Quantification Bottom Fraction Cholesterol HDL Cholesterol LDL Cholesterol
• Source:
  Clin Chim Acta. 431:288-293
• Pubmed ID:
  24589772
• Pubmed Central ID:
  PMC5700747
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  431
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:6d0b89cfbd4e8f6ee116602b1eafbec2a24ffb634b6e817f2bc313c9e55d8c5b
• Download URL:
  https://stacks.cdc.gov/view/cdc/49981/cdc_49981_DS1.pdf
• File Type:
  [PDF - 585.76 KB ]