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Clinical Chemistry and Dried Blood Spots: Increasing Laboratory Utilization by Improved Understanding of Quantitative Challenges
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  1. Development and Implementation Challenges of a Quality Assured HIV Infant Diagnosis Program in Nigeria Using Dried Blood Spots and DNA Polymerase Chain Reaction
    Personal Author:
    Audu, Rosemary; Onwuamah, Chika; Salu, Olumuyiwa; Okwuraiwe, Azuka; Ou, Chin-Yih; Bolu, Omotayo; Bond, Kyle B.; Diallo, Karidia; Lu, Lydia; Jelpe, Tapdiyel; Okoye, McPaul; Ngige, Evelyn; Vertefeuille, John;
    Published:
    Dec 17 2014
    Source:
    AIDS Res Hum Retroviruses. 31(4):433-438.
    Description:
    Nigeria has one of the highest HIV burdens as well as mother-to-infant transmission rates in the world. A pilot program using polymerase chain reaction (PCR)-based testing of dried blood spot (DBS) specimens was implemented to enable early identifica...
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  2. Stabilities of hemoglobins A and S in dried blood spots stored under controlled conditions
    Personal Author:
    Adam, Barbara W.; Chafin, Dana L.; De Jesús, Victor R.;
    Published:
    May 20 2013
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    Clin Biochem. 46(12):1089-1092.
    Description:
    Objective : We aimed to measure separately the contributions of heat and humidity to changes in levels of hemoglobins A and S in dried-blood-spot (DBS) samples. : Design and methods : We stored paired sets of DBSs at 37 °C for predetermined interval...
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  3. Bridging the Gap between Sample Collection and Laboratory Analysis: Using Dried Blood Spots to Identify Human Exposure to Chemical Agents
    Personal Author:
    Hamelin, Elizabeth I.; Blake, Thomas A.; Perez, Jonas W.; Crow, Brian S.; Shaner, Rebecca L.; Coleman, Rebecca M.; Johnson, Rudolph C.;
    Published:
    May 13 2016
    Source:
    Proc SPIE Int Soc Opt Eng. 98630:98630P-98630P-9.
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    Public health response to large scale chemical emergencies presents logistical challenges for sample collection, transport, and analysis. Diagnostic methods used to identify and determine exposure to chemical warfare agents, toxins, and poisons tradi...
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  4. Validation of biomarkers of CVD risk from dried blood spots in community-based research: Methodologies and study-specific serum equivalencies
    Personal Author:
    Samuelsson, Laura B.; Hall, Martica H.; McLean, Shakir; Porter, James H.; Berkman, Lisa; Marino, Miguel; Sembajwe, Grace; McDade, Thomas W.; Buxton, Orfeu M.;
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    2015
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    Biodemography Soc Biol. 61(3):285-297.
    Description:
    Objective : Dried blood spot (DBS) methodology offers significant advantages over venipuncture in vulnerable populations or large-scale studies, including reduced participant burden and higher response rates. Uncertainty about validity of cardiovascu...
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  5. Incident Infection and Resistance Mutation Analysis of Dried Blood Spots Collected in a Field Study of HIV Risk Groups, 2007-2010
    Personal Author:
    Wei, Xierong; Smith, Amanda J.; Forrest, David W.; Cardenas, Gabriel A.; Beck, Dano W.; LaLota, Marlene; Metsch, Lisa R.; Sionean, Catlainn; Owen, S. Michele; Johnson, Jeffrey A.;
    Published:
    Jul 14 2016
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    PLoS One. 11(7).
    Description:
    Objective : To assess the utility of cost-effective dried blood spot (DBS) field sampling for incidence and drug resistance surveillance of persons at high risk for HIV infection. : Methods : We evaluated DBS collected in 2007–2010 in non-clinical ...
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