Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin

Details

• Alternative Title:
  Anal Chim Acta
• Personal Author:
  Hua, Xiude ; Zhou, Liangliang ; Feng, Lu ; Ding, Yuan ; Shi, Haiyan ; Wang, Limin ; Gee, Shirley J. ; Hammock, Bruce D. ; Wang, Minghua
• Description:
  Twenty-three phage-displayed peptides that specifically bind to an anti-benzothiostrobin monoclonal antibody (mAb) in the absence or presence of benzothiostrobin were isolated from a cyclic 8-residue peptide phage library. Competitive and noncompetitive phage enzyme linked immunosorbent assays (ELISAs) for benzothiostrobin were developed by using a clone C3-3 specific to the benzothiostrobin-free mAb and a clone N6-18 specific to the benzothiostrobin immunocomplex, respectively. Under the optimal conditions, the half maximal inhibition concentration (IC50) of the competitive phage ELISA and the concentration of analyte producing 50% saturation of the signal (SC50) of the noncompetitive phage ELISA for benzothiostrobin were 0.94 and 2.27 ng mL(-1), respectively. The noncompetitive phage ELISA showed higher selectivity compared to the competitive. Recoveries of the competitive and the noncompetitive phage ELISAs for benzothiostrobin in cucumber, tomato, pear and rice samples were 67.6-119.6% and 70.4-125.0%, respectively. The amounts of benzothiostrobin in the containing incurred residues samples detected by the two types of phage ELISAs were significantly correlated with that detected by high-performance liquid chromatography (HPLC).
• Subjects:
  • Article
  • Benzothiostrobin
  • Enzyme-linked immunosorbent assay
  • Phage-displayed peptide
  • Phage anti-immunocomplex assay
  • Noncompetitive immunoassay
  • Peptidomimetics
  • Acrylates
  • Antibodies, Monoclonal
  • Benzothiazoles
  More +
• Source:
  Anal Chim Acta. 890:150-156
• Pubmed ID:
  26347177
• Pubmed Central ID:
  PMC4791039
• Document Type:
  Journal Article
• Funding:
  P42 ES004699/ES/NIEHS NIH HHSUnited States/ ; U50 OH007550/OH/NIOSH CDC HHSUnited States/ ; 2U50 OH007550/OH/NIOSH CDC HHSUnited States/ ; P42 ES04699/ES/NIEHS NIH HHSUnited States/
• Volume:
  890
• Download URL:
  https://stacks.cdc.gov/view/cdc/38437/cdc_38437_DS1.pdf
• File Type:
  [PDF - 613.56 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:4b11596d8fd063345f7b07ba6e4167b345450b1cad45347d3308766e4cfcdb59