Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin

Details

• Personal Author:
  Ding Y ; Feng L ; Gee SJ ; Hammock BD ; Hua X ; Shi H ; Wang L ; Wang M ; Zhou L
• Description:
  Twenty-three phage-displayed peptides that specifically bind to an anti-benzothiostrobin monoclonal antibody (mAb) in the absence or presence of benzothiostrobin were isolated from a cyclic 8-residue peptide phage library. Competitive and noncompetitive phage enzyme linked immunosorbent assays (ELISAs) for benzothiostrobin were developed by using a clone C3-3 specific to the benzothiostrobin-free mAb and a clone N6-18 specific to the benzothiostrobin immunocomplex, respectively. Under the optimal conditions, the half maximal inhibition concentration (IC50) of the competitive phage ELISA and the concentration of analyte producing 50% saturation of the signal (SC50) of the noncompetitive phage ELISA for benzothiostrobin were 0.94 and 2.27 ng mL(-1), respectively. The noncompetitive phage ELISA showed higher selectivity compared to the competitive. Recoveries of the competitive and the noncompetitive phage ELISAs for benzothiostrobin in cucumber, tomato, pear and rice samples were 67.6-119.6% and 70.4-125.0%, respectively. The amounts of benzothiostrobin in the containing incurred residues samples detected by the two types of phage ELISAs were significantly correlated with that detected by high-performance liquid chromatography (HPLC). [Description provided by NIOSH]
• Subjects:
  Enzymes Occupational Health Safety
• Keywords:
  Analytical Processes Author Keywords: Benzothiostrobin Enzyme-linked Immunosorbent Assay Immunoassays Noncompetitive Immunoassay Peptidomimetics Phage-displayed Peptide Phage Anti-immunocomplex Assay
• ISSN:
  0003-2670
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  150-156
• Volume:
  890
• NIOSHTIC Number:
  nn:20047692
• Citation:
  Anal Chim Acta 2015 Aug; 890:150-156
• Contact Point Address:
  Minghua Wang, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
• Email:
  wangmha@njau.edu.cn
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Analytica Chimica Acta
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:78924feddef33832f5253240235a49789a1221016f9bf30efe3df053e1135002efe25e33f6322037e2162ae1f1633a967c972285c495bcf611763259630a2f11
• Download URL:
  https://stacks.cdc.gov/view/cdc/202695/cdc_202695_DS1.pdf
• File Type:
  [PDF - 1.01 MB ]