i
Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease
-
11 2015
Source: Nat Biotechnol. 33(11):1159-1161 -
Alternative Title:Nat Biotechnol
-
Personal Author:
-
Description:We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active Streptococcus pyogenes Cas9 nuclease, without inducing double-stranded breaks. We use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells.
-
Subjects:
-
Source:
-
Pubmed ID:26436575
-
Pubmed Central ID:PMC4747789
-
Document Type:
-
Funding:
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
-
pdf xml gif jpeg gif jpeg
Details:
Supporting Files
More +