Details:

Alternative Title:
Toxicol Appl Pharmacol
Personal Author:
Zaccone, Eric J. ; Goldsmith, W. Travis ; Shimko, Michael J. ; Wells, J.R. ; Schwegler-Berry, Diane ; ... More +
Zaccone, Eric J. ; Goldsmith, W. Travis ; Shimko, Michael J. ; Wells, J.R. ; Schwegler-Berry, Diane ; Willard, Patsy A. ; Case, Shannon L. ; Thompson, Janet A. ; Fedan, Jeffrey S. Less -
Description:
Inhalation of butter flavoring by workers in the microwave popcorn industry may result in “popcorn workers' lung.” In previous in vivo studies rats exposed for 6 h to vapor from the flavoring agents, diacetyl and 2,3-pentanedione, acquired flavoring concentration-dependent damage of the upper airway epithelium and airway hyporeactivity to inhaled methacholine. Because ion transport is essential for lung fluid balance,we hypothesized that alterations in ion transport may be an early manifestation of butter flavoring-induced toxicity.We developed a system to expose cultured human bronchial/tracheal epithelial cells (NHBEs) to flavoring vapors. NHBEs were exposed for 6 h to diacetyl or 2,3-pentanedione vapors (25 or ≥ 60 ppm) and the effects on short circuit current and transepithelial resistance (Rt) were measured. Immediately after exposure to 25 ppm both flavorings reduced Na+ transport,without affecting Cl- transport or Na+,K+-pump activity. Rt was unaffected. Na+ transport recovered 18 h after exposure. Concentrations (100-360 ppm) of diacetyl and 2,3-pentanedione reported earlier to give rise in vivo to epithelial damage, and 60 ppm, caused death of NHBEs 0 h post-exposure. Analysis of the basolateral medium indicated that NHBEs metabolize diacetyl and 2,3-pentanedione to acetoin and 2-hydroxy-3-pentanone, respectively. The results indicate that ion transport is inhibited transiently in airway epithelial cells by lower concentrations of the flavorings than those that result in morphological changes of the cells in vivo or in vitro.
Subjects:
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2,3-Pentanedione Article Bronchi Butter Cells, Cultured Diacetyl Dicarbonyl/l-xylulose Reductase Epithelial Cells Flavoring Agents Human Airway Epithelial Cells Humans Inhalation Exposure Ion Transport Methacholine Chloride Microwaves Occupational Exposure Pentanones Vapor Exposure
Source:
Toxicol Appl Pharmacol. 289(3):542-549
Pubmed ID:
26454031
Pubmed Central ID:
PMC4739639
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States ; T32 HL090610/HL/NHLBI NIH HHS/United States ; 5T32HL090610-05/HL/NHLBI NIH HHS/United States
CC999999/Intramural CDC HHS/United States ; T32 HL090610/HL/NHLBI NIH HHS/United States ; 5T32HL090610-05/HL/NHLBI NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:8f7683bd75604ac792aca86c28dc95d9ef91c21d75a2d09a1ce135853568fa8b
Download URL:
https://stacks.cdc.gov/view/cdc/37861/cdc_37861_DS1.pdf
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