Details:

Alternative Title:
Anal Bioanal Chem
Publisher's site:
http://dx.doi.org/10.1007/s00216-014-7907-4
Personal Author:
Sommer, Yuliya L. ; Verdon, Carl P. ; Fresquez, Mark R. ; Ward, Cynthia D. ; Wood, Elliott B. ; ... More +
Sommer, Yuliya L. ; Verdon, Carl P. ; Fresquez, Mark R. ; Ward, Cynthia D. ; Wood, Elliott B. ; Pan, Yi ; Caldwell, Kathleen L. ; Jones, Robert L. ; Less -
Description:
The measurement of different mercury compounds in human blood can provide valuable information about the type of mercury exposure. To this end, our laboratory developed a biomonitoring method for the quantification of inorganic (iHg), methyl (MeHg), and ethyl (EtHg) mercury in whole blood using a triple-spike isotope dilution (TSID) quantification method employing capillary gas chromatography (GC) and inductively coupled dynamic reaction cell mass spectrometry (ICP-DRC-MS). We used a robotic CombiPAL(®) sample handling station featuring twin fiber-based solid-phase microextraction (SPME) injector heads. The use of two SPME fibers significantly reduces sample analysis cycle times making this method very suitable for high sample throughput, which is a requirement for large public health biomonitoring studies. Our sample preparation procedure involved solubilization of blood samples with tetramethylammonium hydroxide (TMAH) followed by the derivatization with sodium tetra(n-propyl)borate (NaBPr(4)) to promote volatility of mercury species. We thoroughly investigated mercury species stability in the blood matrix during the course of sample treatment and analysis. The method accuracy for quantifying iHg, MeHg, and EtHg was validated using NIST standard reference materials (SRM 955c level 3) and the Centre de Toxicologie du Québec (CTQ) proficiency testing (PT) samples. The limit of detection (LOD) for iHg, MeHg, and EtHg in human blood was determined to be 0.27, 0.12, and 0.16 μg/L, respectively.
Subject:
[+]
Article
Biomonitoring
Blood
GC
ICP-MS
Isotope Dilution
Mercury
Speciation
SPME

Source:
Anal Bioanal Chem. 406(20):5039-5047. ;
Pubmed ID:
24948088
Pubmed Central ID:
PMC4685456
Document Type:
Journal Article ;
Funding:
CC999999/Intramural CDC HHS/United States ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:86a6f2383a81c2777463be476c883a0bf30d0b61c6b486159647e49eced02123
File Type:

Supporting Files

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