Characterization of extended-spectrum cephalosporin resistant Salmonella enterica serovar Heidelberg isolated from food animals, retail meat, and humans in the United States 2009

Details

• Alternative Title:
  Foodborne Pathog Dis
• Personal Author:
  Folster, J. P. ; Pecic, G. ; Singh, A. ; Duval, B. ; Rickert, R. ; Ayers, S. ; Abbott, J. ; McGlinchey, B. ; Bauer-Turpin, J. ; Haro, J. ; Hise, K. ; Zhao, S. ; Fedorka-Cray, P. J. ; Whichard, J. ; McDermott, P. F.
• Description:
  Salmonella enterica is one of the most common causes of foodborne illness in the United States. Although salmonellosis is usually self-limiting, severe infections typically require antimicrobial treatment, and ceftriaxone, an extended-spectrum cephalosporin (ESC), is commonly used in both adults and children. Surveillance conducted by the National Antimicrobial Resistance Monitoring System (NARMS) has shown a recent increase in ESC resistance among Salmonella Heidelberg isolated from food animals at slaughter, retail meat, and humans. ESC resistance among Salmonella in the United States is usually mediated by a plasmid-encoded bla(CMY) β-lactamase. In 2009, we identified 47 ESC-resistant bla(CMY)-positive Heidelberg isolates from humans (n=18), food animals at slaughter (n=16), and retail meats (n=13) associated with a spike in the prevalence of this serovar. Almost 90% (26/29) of the animal and meat isolates were isolated from chicken carcasses or retail chicken meat. We screened NARMS isolates for the presence of bla(CMY), determined whether the gene was plasmid-encoded, examined pulsed-field gel electrophoresis patterns to assess the genetic diversities of the isolates, and categorized the bla(CMY) plasmids by plasmid incompatibility groups and plasmid multi-locus sequence typing (pMLST). All 47 bla(CMY) genes were found to be plasmid encoded. Incompatibility/replicon typing demonstrated that 41 were IncI1 plasmids, 40 of which only conferred bla(CMY)-associated resistance. Six were IncA/C plasmids that carried additional resistance genes. pMLST of the IncI1-bla(CMY) plasmids showed that 27 (65.8%) were sequence type (ST) 12, the most common ST among bla(CMY)-IncI1 plasmids from Heidelberg isolated from humans. Ten plasmids had a new ST profile, ST66, a type very similar to ST12. This work showed that the 2009 increase in ESC resistance among Salmonella Heidelberg was caused mainly by the dissemination of bla(CMY) on IncI1 and IncA/C plasmids in a variety of genetic backgrounds, and is likely not the result of clonal expansion.
• Subjects:
  Adult Animals Anti-Bacterial Agents Beta-Lactamases Cephalosporin Resistance Cephalosporins Chickens DNA, Bacterial Drug Resistance, Multiple, Bacterial Electrophoresis, Gel, Pulsed-Field Female Genetic Variation Humans Male Meat Microbial Sensitivity Tests Multilocus Sequence Typing Plasmids Salmonella Enterica Salmonella Food Poisoning

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• Source:
  Foodborne Pathog Dis. 9(7):638-645.
• Pubmed ID:
  22755514
• Pubmed Central ID:
  PMC4620655
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Place as Subject:
  United States
• Volume:
  9
• Issue:
  7
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:3ff521dad48c77c248c3446a3f204cabc426ca11a2595fbb2279a9378bc861ae
• Download URL:
  https://stacks.cdc.gov/view/cdc/35351/cdc_35351_DS1.pdf
• File Type:
  [PDF - 416.34 KB ]