Chronic fetal hypoxia affects axonal maturation in guinea pigs during development: a longitudinal Diffusion Tensor Imaging and T2 mapping study

Kim, Jieun; Choi, In-Young; Dong, Yafeng; Wang, Wen-Tung; Brooks, William M.; Weiner, Carl P.; Lee, Phil

doi:10.1002/jmri.24825

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Chronic fetal hypoxia affects axonal maturation in guinea pigs during development: a longitudinal Diffusion Tensor Imaging and T2 mapping study

Published Date:

Dec 15 2014

Publisher's site:

http://dx.doi.org/10.1002/jmri.24825 New Site Icon

Source:

J Magn Reson Imaging. 42(3):658-665.

[PDF-1.71 MB]

Details:

Personal Authors:

Kim, Jieun ; Choi, In-Young ; Dong, Yafeng ; Wang, Wen-Tung ; Brooks, William M. ; ... More ▼

Keywords:

[+]

Pubmed ID:

25504885

Pubmed Central ID:

PMC4468050

Description:

Purpose

Chronic hypoxemia is the prime cause of fetal brain injury and long-term sequelae such as neurodevelopmental compromise, seizures and cerebral palsy. This study aims to investigate the impact of chronic hypoxemia on neonatal brains, and follow developmental alterations and adaptations non-invasively in a guinea pig model.

Materials and Methods

Thirty guinea pigs underwent either normoxic and hypoxemic conditions during the critical stage of brain development (0.7 gestation) and studied prenatally (n=16) or perinatally (n=14). Fourteen newborns (7 hypoxia and 7 normoxia group) were scanned longitudinally to characterize physiological and morphological alterations, and axonal myelination and injury using in vivo DTI, T2 mapping, and T2-weighted MRI. Sixteen fetuses (8 hypoxia and 8 normoxia) were studied ex vivo to assess hypoxia-induced neuronal injury/loss using Nissl staining and quantitative reverse transcriptase Polymerase Chain Reaction methods.

Results

Developmental brains in the hypoxia group showed lower fractional anisotropy in the corpus callosum (−12%, p=0.02) and lower T2 values in the hippocampus (−16%, p=0.003) compared with the normoxia group with no differences in the cortex (p>0.07), indicating vulnerability of the hippocampus and cerebral white matter during early development. Fetal guinea pig brains with chronic hypoxia demonstrated an over-tenfold increase in expression levels of hypoxia index genes such as erythropoietin and HIF-1α, and an over 40% reduction in neuronal density, confirming prenatal brain damage.

Conclusion

In vivo MRI measurement, such as DTI and T2 mapping, provides quantitative parameters to characterize neuro-developmental abnormalities and to monitor the impact of prenatal insult on the postnatal brain maturation of guinea pigs.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

Supporting Files:

	nihms647426f4.jpg	image/jpeg
	nihms647426f5.gif	image/gif
	nihms647426f5.jpg	image/jpeg
	nihms647426.nxml	application/octet-stream
	nihms647426f1.gif	image/gif
	nihms647426f1.jpg	image/jpeg
	nihms647426f2.gif	image/gif
	nihms647426f2.jpg	image/jpeg
	nihms647426f3.gif	image/gif
	nihms647426f3.jpg	image/jpeg
	nihms647426f4.gif	image/gif

No Related Documents.

You May Also Like:

Maximum Principal Strain and Strain Rate Associated with Concussion Diagnosis Correlates with Changes in Corpus Callosum White Matter Indices

Personal Author:

MCALLISTER, THOMAS W.; FORD, JAMES C.; JI, SONGBAI; BECKWITH, JONATHAN G.; FLASHMAN, LAURA A.; PAULSEN, KEITH; GREENWALD, RICHARD M.;

Published:

Oct 13 2011

Source:

Ann Biomed Eng. 2011; 40(1):127-140.

Description:

On-field monitoring of head impacts, combined with finite element (FE) biomechanical simulation, allow for predictions of regional strain associated with a diagnosed concussion. However, attempts to correlate these predictions with in vivo measures o...

File Type:

[PDF - 1.58 MB]
Plasticity of the human visual system after retinal gene therapy in patients with Leber’s congenital amaurosis

Personal Author:

Ashtari, Manzar; Zhang, Hui; Cook, Philip A.; Cyckowski, Laura L.; Shindler, Kenneth S.; Marshall, Kathleen A.; Aravand, Puya; Vossough, Arastoo; Gee, James C.; Maguire, Albert M.; Baker, Chris I.; Bennett, Jean;

Published:

Jul 15 2015

Source:

Sci Transl Med. 7(296):296ra110.

Description:

Much of our knowledge of the mechanisms underlying plasticity in the visual cortex in response to visual impairment, vision restoration, and environmental interactions comes from animal studies. We evaluated human brain plasticity in a group of patie...

File Type:

[PDF - 854.46 KB]
Diffusion Tensor Imaging Reliably Differentiates Patients With Schizophrenia from Healthy Volunteers

Personal Author:

Ardekani, Babak A.; Tabesh, Ali; Sevy, Serge; Robinson, Delbert G.; Bilder, Robert M.; Szeszko, Philip R.;

Published:

Jan 2011

Source:

Hum Brain Mapp. 32(1):1-9.

Description:

The objective of this research was to determine whether fractional anisotropy (FA) and mean diffusivity (MD) maps derived from diffusion tensor imaging (DTI) of the brain are able to reliably differentiate patients with schizophrenia from healthy vol...

File Type:

[PDF - 518.99 KB]
A multimodal imaging analysis of subcortical gray matter in fragile X premutation carriers

Personal Author:

Wang, Jun Yi; Hagerman, Randi J.; Rivera, Susan M.;

Published:

May 06 2013

Source:

Mov Disord. 28(9):1278-1284.

Description:

Background : Approximately 40% of males with the fragile X premutation develop fragile X-associated tremor/ataxia syndrome after age 50. Although the thalamus and basal ganglia play a crucial role in movement disorders, their involvement in fragile X...

File Type:

[PDF - 1.14 MB]
Thalamocortical Dysfunction and Thalamic Injury after Asphyxial Cardiac Arrest in Developing Rats

Personal Author:

Shoykhet, Michael; Simons, Daniel J.; Alexander, Henry; Hosler, Christina; Kochanek, Patrick M.; Clark, Robert S. B.;

Published:

Apr 4 2012

Source:

J Neurosci. 32(14):4972-4981.

Description:

Global hypoxia-ischemia interrupts oxygen delivery and blood flow to the entire brain. Previous studies of global brain hypoxia-ischemia have primarily focused on injury to the cerebral cortex and to the hippocampus. Susceptible neuronal populations ...

File Type:

[PDF - 5.63 MB]